黄颡鱼养殖群体遗传多样性与遗传结构的微卫星分析  

Genetic diversity and genetic structure analysis of Pelteobagrus fulvidraco using microsatellite markers

作　　者：李燕平 覃川杰[1] 吕云云 龚全[2] 王均 颉江[1] 文正勇 LI Yan-ping;QIN Chuan-jie;LV Yun-yun;GONG Quan;WANG Jun;XIE Jiang;WEN Zheng-yong(Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River,Neijiang Normal University,Neijiang 641112,Sichuan,China;Institute of Fisheries,Sichuan Academy of Agricultural Sciences,Chengdu 611730,China)

机构地区：[1]内江师范学院,长江上游鱼类资源保护与利用四川省重点实验室,四川内江641112 [2]四川省农业科学院水产研究所,成都611730

出　　处：《淡水渔业》2023年第3期3-14,共12页Freshwater Fisheries

基　　金：高品质鱼类育种材料和方法创新及新品种选育(2021YFYZ0015);省级现代水产种业园区培育项目育种创新能力建设;内江师范学院重点项目(2021ZD09)。

摘　　要：为研究14个不同养殖群体的黄颡鱼(Pelteobagrus fulvidraco)遗传多样性及遗传结构,采用8对多态性微卫星引物对14个群体的209尾个体进行遗传多样性和遗传结构分析。结果显示:14个群体的平均等位基因数在8~10之间,平均有效等位基因在5.160~6.882之间,平均观测杂合度在0.633~0.717之间,平均期望杂合度在0.358~0.749之间,平均多态信息含量在0.594~0.681之间,均大于0.5。分子方差分析结果表明:3.69%的遗传变异来自群体间,3.93%来自群体内个体间,92.38%来自所有个体间,变异来源主要来自个体间,其中RB群体和WH群体的遗传分化最大(F_(st)=0.116),ZL群体和XB群体的遗传分化最小(F_(st)=-0.009)。基于遗传距离构建了UPGMA系统树,发现14个群体共分为5个聚类支,HJ、YZ、YH、YB 4个群体聚为一支,HQ和LG单独各自聚为一支,LZ和RB 2个群体聚为一支,DB、ZJ、WH、XH、XB和ZL 6个群体聚为一支。基于贝叶斯的Structure聚类分析同样支持14个黄颡鱼群体可分为5个聚类支,与聚类树的结果吻合。14个黄颡鱼群体的遗传多样性均处于高度多态水平,遗传分化处于低等分化水平。Pelteobagrus fulvidraco,a member of the family Bagridaein the Siluriformesis a crucial fish for China′s economy and primary breeding stock.It is widely dispersedalong the Yangtze River,Yellow River,Pearl River,Yuan River and Heilongjiang River.Due to inbreeding,extensive trade,breeding and release,and natural escape,the population′s genetic diversity might be reduced.Recent analyses of the genetic diversity and structure of P.fulvidraco have mainly concentrated on wild populations.Additionally,obtaining information on the natural populations is challenging because of the Yangtze River′s ten-year fishing ban plan,which is currently in effect.The P.fulvidraco artificial breeding population′s genetic diversity and structure are therefore essential to understand and master to provide a theoretical foundation for the selection of improved seeds and seedling propagation.Simple sequence repeats(SSRs),also known as microsatellites,were frequently used in population genetic analyses due to their higher levels of polymorphism,codominance,and abundance in the genome than conventional molecular markers.In the current study,a total of 209 individuals from 14 P.fulvidraco aquaculture populations were genetically analyzed using eight microsatellite loci to assess genetic diversity.This analysisprovided data support for family establishment,shortened breeding life,and acceleratedthe selection of new species for P.fulvidraco.The findingsindicatedthatthe mean number of alleles(Na)ranging from 8 to 10,the average number of effective alleles(Ne)rangingfrom 5.160 to 6.822.The average observed heterozygosity(Ho)ranged ranging from 0.633 to 0.717,the average expected heterozygosity(He)went ranging from 0.358 to 0.749.The average polymorphism information content(PIC)ranged from 0.594 to 0.681,implying high polymorphism in all of the 14 populations(PIC>0.5).Genetic differentiation among populations revealedthat RB and WH populations had the highest level of differentiation level(F st=0.116).In contrast,ZL and XB populationshad th

关 键 词：黄颡鱼(Pelteobagrus fulvidraco) 养殖群体 微卫星标记 遗传多样性 

分 类 号：S917[农业科学—水产科学]