扁桃酸对肺腺癌H1299细胞增殖、凋亡和迁移能力的影响及其机制  

Effect of mandelic acid on the proliferation,apoptosis and migration ability of lung adenocarcinoma H1299 cells and its mechanism

作　　者：陆海青 李艳丽 华兹涵 肖佳欣 凌博 叶广彬 Lu Haiqing;Li Yanli;Hua Zihan;Xiao Jiaxin;Ling Bo;Ye Guangbin(School of Pharmacy,YouJiang Medical University for Nationalities,Baise 533000;School of Basic Medical Sciences,YouJiang Medical University for Nationalities,Baise 533000)

机构地区：[1]右江民族医学院药学院,百色533000 [2]右江民族医学院基础医学院,百色533000

出　　处：《安徽医科大学学报》2023年第5期742-747,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82060540);广西高校中青年教师基础能力提升项目(编号:2022KY0541、2022KY0533);广西壮族自治区级大学生创新创业训练计划项目(编号:S202210599069、S202110599069、S202110599095)。

摘　　要：目的探索扁桃酸对肺腺癌H1299细胞增殖、凋亡和迁移的作用及相关的分子机制。方法CCK-8检测H1299细胞增殖能力变化;Hoechst 33258/PI双染分析H1299细胞凋亡水平;划痕和Transwell实验分析H1299细胞侵袭迁移能力变化;Western blot检测细胞增殖、凋亡和迁移相关通路蛋白的表达。结果不同浓度扁桃酸均可抑制H1299细胞增殖活力和侵袭迁移能力(P<0.05)。扁桃酸可诱导细胞增殖、凋亡通路蛋白bax、cl-caspase-3高表达,p-stat3低表达(P<0.05)。此外,抑制侵袭迁移相关蛋白MMP-9和Vimentin蛋白表达(P<0.01)。结论扁桃酸抑制肺腺癌细胞H1299的增殖,提升细胞凋亡水平,其分子生物学机制可能与stat3活化降低及激活bax/caspase-3信号轴密切相关;抑制H1299细胞侵袭迁移能力,与MMP-9和Vimentin蛋白表达降低相关。Objective The research was aimed at exploring the effects of mandelic acid on the proliferation,apoptosis and migration of H1299 lung adenocarcinoma(LUAD)cells and the related molecular mechanisms.Methods CCK-8 was used to detect changes in the proliferation ability of H1299 cells;Hoechst 33258/PI double-staining assay was used to analyze the apoptosis of H1299 cells;Transwell and migration assays were used to analyze changes in the invasive and migration ability of H1299 cells;Western blot was used to detect the expression of proliferation,apoptosis and migration-related pathway proteins.Results Different concentrations of mandelic acid inhibited the proliferation viability and invasion and migration ability of H1299 cells(P<0.05).Mandelic acid induced high expression of bax and cl-caspase-3 and low expression of p-stat3 in proliferation and apoptosis pathways(P<0.05).In addition,it inhibited the expression of MMP-9 and Vimentin proteins,which were related to invasion and migration(P<0.01).Conclusion Mandelic acid inhibited the proliferation of H1299 LUAD cells and elevated the level of apoptosis,and molecular biological mechanism might be closely related to the decreased activation of stat3 and activation of bax/caspase-3 signaling axis.The inhibition of invasive and migrational ability of H1299 cells induced by mandelic acid was associated with the decreased expression of MMP-9 and Vimentin proteins.

关 键 词：肺腺癌 扁桃酸 增殖 凋亡 迁移 

分 类 号：R932[医药卫生—生药学]