直接靶板微滴法快速检测肺炎克雷伯菌替加环素和多黏菌素的敏感性  

A rapid tigecycline and polymyxin B susceptibility test for Klebsiella pneumoniae using direct-on-target micro-droplet growth assay

作　　者：夏兆新 杨文苏 朱毅 胡心益 林春晖 蒋童 沈继录[1] Xia Zhaoxin;Yang Wensu;Zhu Yi;Hu Xinyi;Lin Chunhui;Jiang Tong;Shen Jilu(The First Affiliated Hospital of Anhui Medical University,The Anhui Public Health Clinical Center,Laboratory Dept,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院检验科,安徽省公共卫生临床中心,合肥230022

出　　处：《安徽医科大学学报》2023年第5期859-862,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高等学校自然科学研究重大项目(编号:KJ2021ZD0032)。

摘　　要：目的探究直接靶板微滴法(DOT-MGA)快速检测替加环素和多黏菌素敏感性。方法共收集了67株肺炎克雷伯菌进行DOT-MGA测试,将有或没有替加环素、多黏菌素(药物终浓度为2μg/ml)的6μl微滴一式三份加在靶板上,设置了3、4、6、8 h四个孵育时间点。Bruker Biotyper软件将测试结果判定为敏感(评分<1.7分)或非敏感(评分≥1.7分)。结果孵育4 h后,替加环素和多黏菌素的生长有效率、特异度、阳性预测值均为100.00%,分类一致率分别为98.15%、96.15%,灵敏度分别为96.30%、92.31%,阴性预测值分别为96.45%、92.86%。结论DOT-MGA可以快速准确鉴别对替加环素和多黏菌素的耐药表型,与微量肉汤稀释法比较具有良好的一致性。Objective To explore the direct-on-target micro-droplet growth assay(DOT-MGA)for rapid detection of the susceptibility of tigecycline and polymyxin.Methods A total of 67 strains of Klebsiella pneumoniae were collected for DOT-MGA.6μl droplets with or without tigecycline or polymyxin(The final drug concentration is 2μg/ml)were added in triplicate into the wells of the MALDI plate with four incubation time points(3 h,4 h,6 h,and 8 h).The results were classified as susceptible(score<1.7)and non-susceptible(score≥1.7)according to the Bruker Biotype software.Results After incubation for 4 h,the growth efficiency,specificity and positive predictive value of tigecycline and polymyxin were both 100.00%.The classification consistency rate was 98.15%and 96.15%,the sensitivity was 96.30%and 92.31%,and the negative predictive value was 96.45%and 92.86%,respectively.Conclusion DOT-MGA can provide rapid and reliable drug susceptibility diagnosis of tigecycline and polymyxin,which is of great significance for the clinical anti-infective treatment.

关 键 词：直接靶板微滴法 快速药敏试验 替加环素 多黏菌素 

分 类 号：R446.5[医药卫生—诊断学]