金黄垂直链霉菌DF06的分类鉴定及防病促生作用  

Classification and Identification of Streptomyces aureoverticillatus DF06 and Its Effect on Disease Control and Growth Promotion

作　　者：石义妃 耿佩冰 吴皓 苗建强 韩立荣 刘西莉 SHI Yifei;GENG Peibing;WU Hao;MIAO Jianqiang;HAN Lirong;LIU Xili(College of Forestry and Plant Protection,Northwest A&F University,Yangling 712100,China;College of Forestry,Northwest A&F University,Yangling 712100,China)

机构地区：[1]西北农林科技大学植物保护学院,杨凌712100 [2]西北农林科技大学林学院,杨凌712100

出　　处：《中国生物防治学报》2023年第2期407-417,共11页Chinese Journal of Biological Control

基　　金：西安市科技计划项目(20NYYF0025);渭南市科技计划项目(2022ZDYFJH-209)。

摘　　要：为研究生防菌株DF06对黄瓜枯萎病的防效和生防机制,通过培养特征、生理生化特征以及16S rDNA序列分析对DF06进行分类鉴定;采用平板对峙和室内盆栽法测定DF06对黄瓜枯萎病菌菌丝生长的抑制作用、对病害的防治效果及对黄瓜植株促生作用和防御酶活性变化影响;并通过实时荧光定量技术测定抗性相关基因的表达。结果表明:菌株DF06为金黄垂直链霉菌Streptomycesaureoverticillatus,该菌株对黄瓜枯萎病病原菌有较强的抑制作用,菌丝抑制率达80.80%,且能够导致黄瓜枯萎病菌基内菌丝发生畸变;在室内盆栽试验中DF06发酵液对黄瓜枯萎病有较好的防治效果,其保护作用达到68.42%,优于对照药剂多菌灵;不同浓度DF06发酵液能促进黄瓜种子和幼苗生长,经107CFU/mL的DF06发酵液处理后黄瓜幼苗株高、叶绿素含量和干重均显著增加,分别可达23.56 cm、2.16 mg/g和0.84 g;同时该菌能够诱导黄瓜抗病相关基因表达量的上调,提高相关防御酶活性,其中CAT、PAL和POD活性均在处理后的24h内快速增加。研究表明,DF06菌株可通过分泌某些拮抗物质直接抑制病原菌基内菌丝生长及诱导植物系统性抗病等方式有效防治黄瓜枯萎病,其作为防治黄瓜枯萎病新的生防菌资源,具有进一步开发价值。This study aimed to investigate control efficiency and biocontrol mechanism of strain DF06 against Fusarium oxysporum f.sp.cucumerinum.DF06 was classified and identified by culture characteristics,physiological and biochemical characteristics and 16S rDNA sequence analysis.The agar plate method was used to determine the antibacterial activity of the strain on the growth of F.oxysporium f.sp.cucumerinum FOC mycelium.The control efficiency of strain DF06 to cucumber disease and its effect on the growth potential and the changes of defense enzymes in cucumber plants were determined by using pot experiments.Real-time quantitative technique was used to determine the resistance-related genes.The results showed that the strain DF06 was identified as Streptomyces aureoverticillatus,which had a strong inhibitory effect on F.oxysporium f.sp.cucumerinum FOC,with the inhibition rate of 80.80%and malformed hyphae.The control efficiency of strain DF06 to cucumber Fusarium wilt was 68.42%,higher than that of the control agent.Different concentrations of DF06 fermentation broth can promote the growth of cucumber seeds and seedlings.The plant height,chlorophyll content and dry weight increased significantly after treatment with 107 CFU/mL of DF06 fermentation broth,reaching to 23.56 cm,2.16 mg/g and 0.84 g,respectively.At the same time,the bacteria induced the up-regulation of the expression of disease resistance-related genes and improved the activities of related defense enzymes in cucumber plants.The activities of CAT,PAL and POD all increased rapidly within 24 h after treatment.DF06 strain can effectively control F.oxysporium f.sp.cucumerinum FOC by directly inhibiting the mycelium growth and inducing the plant resistance to disease.DF06 strain has the potential to be developed into biocontrol agent for the control of cucumber Fusarium wilt.

关 键 词：黄瓜枯萎病 金黄垂直链霉菌 促生作用 生防机制 

分 类 号：S476[农业科学—农业昆虫与害虫防治]