城乡交错区的土壤微生物多样性格局及其影响因素  

Diversity pattern and driving factors for soil microbes in a peri-urban area

作　　者：高燕[1] 王嵘[1] GAO Yan;WANG Rong(Zhejiang Tiantong Forest Ecosystem National Observation and Research Station,East China Normal University,Ningbo,Zhejiang 315114,China)

机构地区：[1]华东师范大学浙江天童森林生态系统国家野外科学观测研究站,浙江宁波315114

出　　处：《华东师范大学学报（自然科学版）》2023年第3期20-32,共13页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(32071667)。

摘　　要：以上海市松江区泖港镇为研究地点,采用高通量测序技术全面揭示了该城乡交错区不同土地利用类型中土壤细菌和土壤真菌的多样性分布格局和群落组成格局.研究结果显示,采集的土壤样品中共发现632种土壤细菌OTUs (operational taxonomic units)和593种土壤真菌OTUs,优势类群为变形菌门(细菌)与子囊菌门(真菌). 4种土地利用类型(耕地、坑塘水域、仓储用地和居民居住区)中,土壤细菌的Shannon指数为4.41~4.71,土壤真菌的Shannon指数为1.99~2.60,这两类土壤微生物的α多样性在各土地利用类型间无显著差异.而不同土地利用类型间,土壤细菌(R=0.32, p=0.001)和土壤真菌(R=0.22, p=0.001)的β多样性格局存在显著差异.此外,该城乡交错区土壤细菌和土壤真菌的群落组成格局和β多样性格局与土壤酸碱度、土壤孔隙度和土壤含水率显著相关.We collected soil samples from Maogang Town(Songjiang District,Shanghai)and used high-throughput sequencing techniques to reveal the diversity and community composition of soil bacteria and soil fungi in this peri-urban area.We found a total of 632 bacteria OTUs(operational taxonomic units)and 593 fungi OTUs in Maogang,in which the dominant microbial groups were Proteobacteria(bacteria)and Ascomycota(fungi).The Shannon Wiener index of soil bacteria was found to be in the range of 4.41 to 4.71,and the soil fungi’s Shannon Wiener index was found to be in the range of 1.99 to 2.60.Land-use type insignificantly affected α diversity of soil bacteria and soil fungi;however,land-use type significantly affected β diversity of soil bacteria(R=0.32,p=0.001)and soil fungi(R=0.22,p=0.001).Lastly,there were significant correlations observed between three soil environmental factors—namely,soil pH,porosity,and moisture—and community composition and β diversity of soil bacteria and soil fungi.

关 键 词：生物多样性 土壤细菌 土壤真菌 城乡交错区 

分 类 号：Q89[生物学]