高通量测序技术在B族链球菌鉴别分型中的研究进展  

Application progress of multiple high-throughput sequencing technologiesin identification and typing of group B Streptococcus

作　　者：易乐晖 王飞玲(综述)[2] 吴丽娟(审校) YI Lehui;WANG Feiling;WU Lijuan(Zunyi Medical University Zhuhai Campus,Zhuhai,Guangdong 519000,China;Department of Clinical Laboratory,Shenzhen Bao′an Women′s and Children′s Hospital,Shenzhen,Guangdong 518106,China;Maternal-Fetal Medicine Institute,Shenzhen Bao′an Women′s and Children′s Hospital,Shenzhen,Guangdong 518106,China)

机构地区：[1]遵义医科大学珠海校区,广东珠海519000 [2]深圳市宝安区妇幼保健院检验科,广东深圳518106 [3]深圳市宝安区妇幼保健院母胎医学研究所,广东深圳518106

出　　处：《国际检验医学杂志》2023年第10期1255-1259,共5页International Journal of Laboratory Medicine

基　　金：国家自然科学基金项目(81902121);深圳市科技创新委员会项目(JCYJ20180228163744684);科技基础资源调查专项(2019FY101200);深圳市医学重点学科建设经费资助项目(SZXK028)。

摘　　要：近年来,多种基于高通量测序技术建立的测序方法如16S核糖体RNA(16S rRNA)测序、宏基因组测序(mNGS)、全基因组测序(WGS)、RNA测序(RNA-seq)和转座子测序(Tn-seq)逐步被应用于病原学研究。B族链球菌(GBS)是导致围生期母婴感染的首位病原菌,其流行病学分型及相关毒力基因不仅与其致病和防控密切相关,而且具备应用于筛查和诊断的潜力。该文着重综述高通量测序技术应用于GBS的鉴别分型的相关研究进展。In recent years,a variety of sequencing methods based on high-throughput sequencing technologies have been gradually used in etiological research,such as 16S ribosomal RNA(rRNA)sequencing,metagenomic next-generation sequencing(mNGS),whole genome sequencing(WGS),RNA sequencing(RNA-seq)and transposon sequencing(Tn-seq).Group B Streptococcus(GBS)is the leading cause of perinatal maternal infection.Its epidemiological typing and related virulence genes are not only closely related to its pathogenicity and prevention,but also have the potential to be applied in screening and diagnosis.This review focuses on the important application progress in identification and typing of GBS by above-mentioned high-throughput sequencing methods.

关 键 词：B族链球菌 高通量测序 鉴别 分型 毒力基因 

分 类 号：R542.2[医药卫生—心血管疾病] R446.5[医药卫生—内科学]