Stereoselective metabolic disruption of cypermethrin by remolding gut homeostasis in rat  

作　　者：Quan Zhang Sijia Gu YanWang Shitao Hu Siqing Yue Cui Wang 

机构地区：[1]Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province,College of Environment,Zhejiang University of Technology,Hangzhou 310032,China [2]School of Life Science,Zhejiang Chinese Medical University,Hangzhou 310053,China

出　　处：《Journal of Environmental Sciences》2023年第4期761-771,共11页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 21777147)。

摘　　要：Cypermethrin(CYP), a prototypical synthetic pyrethroid, reportedly causes metabolic disruption, while its stereoselective impact remains elusive. This study initially revealed that only α-CYP caused significant weight loss at 8.5 mg/(kg·day) in rats. All three CYP isomers caused the accumulation of hepatic glycogen, and hyperlipemia phenotype as the increment of total triglyceride. Rats treated with α-CYP had markedly high blood glucose levels and homeostasis model assessment of insulin resistance index. The systematic inflammation of θ-CYP group rats was evidenced by high lipopolysaccharide-binding protein levels and abnormalities of leukocytes indices. By examining the gut microbiome, we found thatα-CYP-treated rats had low contents of Firmicutes and high levels of Verrucomicrobia while Elusimicrobia was enriched in the β-CYP group. The increasing alpha diversity in the θ-CYP group may be due to the dominance of pathogenic bacteria and the increase of probiotics to counteract adverse effects. Exclusively, the α-CYP group enriched total short-chain fatty acids(SCFAs), whereas most SCFAs depleted in the θ-CYP group. The correlation analysis further found Firmicutes, an energy storage modulator, was positive to body weight(BW),while SCFAs exerted the opposite, confirming the low BW in α-CYP. Blood glucose that correlated well with SCFAs and Verrucomicrobia can be accounted for the discrepancy betweenα-CYP and θ-CYP. Overall, the three isomers exerted stereoselective glycolipid disruption in rats, and gut homeostasis acted as vital indicators.

关 键 词：CYPERMETHRIN Stereoisomeric selectivity Gut microbiota Short-chain fatty acids Glycolipid metabolism 

分 类 号：X592[环境科学与工程—环境工程] X503.1[医药卫生—卫生毒理学] R114[医药卫生—公共卫生与预防医学]