Transcriptome reveals overview of Ca^(2+) dose-dependent metabolism disorders in zebrafish larvae after Cd^(2+) exposure  

作　　者：Biran Zhu Ziniu Wang Lei Lei Yongyong Guo Jian Han Bingsheng Zhou 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China [2]School of Basic Medical Sciences,Hubei University of Chinese Medicine,Wuhan 430065,China [3]State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China

出　　处：《Journal of Environmental Sciences》2023年第3期480-491,共12页环境科学学报（英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA23040401);the National Natural Science Foundation of China (No. 21806091);the Natural Science Foundation of Hubei Province (No. 2020CFB154)。

摘　　要：Cadmium (Cd),a ubiquitous environmental hazardous heavy metal,poses a significant threat to the health of aquatic organisms,including teleosts.Although the toxic profile of Cd is well recognized,little is known regarding the overall view of toxic responses to varying aquatic environmental parameters (e.g.,water hardness) at an individual level.Herein,differences in water hardness were partially mimicked by adjusting Ca^(2+)levels in E3 medium.As an in vivo model,zebrafish embryos were exposed to variable Ca^(2+)levels (NV,normal Ca^(2+);LV,low Ca^(2+);HV,high Ca^(2+)) alone or combined with 30.7μg/L Cd^(2+)(NC,LC,and HC,respectively) until 144 hr post-fertilization.The genome-wide transcriptome revealed differentially expressed genes between groups.Functional enrichment analysis found that biological processes related to metabolism,particularly lipid metabolism,were significantly disrupted in NC and LC treatments,while a remission was observed in the HC group.Biochemical assays confirmed that the decrease in Ca^(2+)enhanced synthesis,inhibited mobilization and increased the storage of lipids in Cd^(2+)treatments.This study suggests that the toxic effect of Cd on biological pathways will be influenced by Ca^(2+),which will improve the toxicological understanding and facilitate accurate assessment of Cd.

关 键 词：Cadmium Calcium Zebrafish larvae TRANSCRIPTOME Metabolism disorder 

分 类 号：X503.225[环境科学与工程—环境工程]