Environmental Mn(Ⅱ) enhances the activity of dissimilatory arsenate-respiring prokaryotes from arsenic-contaminated soils  

作　　者：Yan Wu Weiwei Wu Yifan Xu Yanxia Zuo Xian-Chun Zeng 

机构地区：[1]State Key Laboratory of Biogeology and Environmental Geology&School of Environmental Studies,China University of Geosciences(Wuhan),Wuhan 430074,China [2]Institute of Hydrobiology,the Chinese Academy of Sciences,Wuhan 430072,China

出　　处：《Journal of Environmental Sciences》2023年第3期582-592,共11页环境科学学报（英文版）

基　　金：supported by the General Programs (No. 41472219);the Foundations for Innovative Research Groups (No. 41521001) from the National Natural Science Foundation of China。

摘　　要：Many investigations suggest that dissimilatory arsenate-respiring prokaryotes (DARPs) play a key role in stimulating reductive mobilization of As from solid phase into groundwater,but it is not clear how environmental Mn(Ⅱ) affects the DARPs-mediated reductive mobilization of arsenic.To resolve this issue,we collected soil samples from a realgar tailingsaffected area.We found that there were diverse arsenate-respiratory reductase (arr) genes in the soils.The microbial communities had high arsenate-respiring activity,and were able to efficiently stimulate the reductive mobilization of As.Compared to the microcosms without Mn(Ⅱ),addition of 10 mmol/L Mn(Ⅱ) to the microcosms led to 23.99%-251.79% increases in the microbial mobilization of As,and led to 133.3%-239.2% increases in the abundances of arr genes.We further isolated a new cultivable DARP,Bacillus sp.F11,from the arseniccontaminated soils.It completely reduced 1 mmol/L As(V) in 5 days under the optimal reaction conditions.We further found that it was able to efficiently catalyze the reductive mobilization and release of As from the solid phase;the addition of 2 mmol/L Mn(Ⅱ) led to 98.49%-248.78% increases in the F11 cells-mediated reductive mobilization of As,and70.6%-104.4% increases in the arr gene abundances.These data suggest that environmental Mn(Ⅱ) markedly increased the DARPs-mediated reductive mobilization of As in arseniccontaminated soils.This work provided a new insight into the close association between the biogeochemical cycles of arsenic and manganese.

关 键 词：dissimilatory arsenate-respiring PROKARYOTE Arsenic mobilization Arsenic-contaminated groundwater Arsenate reduction MN(II) 

分 类 号：X53[环境科学与工程—环境工程] X17