Impact of different organic matters on the occurrence of antibiotic resistance genes in activated sludge  

作　　者：Xiuwen Zhang Suwan Liu Haohao Sun Kailong Huang Lin Ye 

机构地区：[1]State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing 210023,China [2]Nanjing Jiangdao Institute of Environmental Research Co.,Ltd.,Nanjing 210019,China

出　　处：《Journal of Environmental Sciences》2023年第5期273-283,共11页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.51878333);the Natural Science Foundation of Jiangsu Province(No.BK20201260).

摘　　要：The occurrence of antibiotic resistance genes(ARGs)in various environments has drawn worldwide attention due to their potential risks.Previous studies have reported that a variety of substances can enhance the occurrence and dissemination of ARGs.However,few studies have compared the response of ARGs under the stress of different organicmatters in biologicalwastewater treatment systems.In this study,seven organic pollutantswere added into wastewater treatment bioreactors to investigate their impacts on the ARG occurrence in activated sludge.Based on high-throughput sequencing,it was found that the microbial communities and ARG patterns were significantly changed in the activated sludge exposed to these organic pollutants.Compared with the non-antibiotic refractory organic matters,antibiotics not only increased the abundance of ARGs but also significantly changed the ARG compositions.The increase of Gram-negative bacteria(e.g.,Archangium,Prosthecobacter and Dokdonella)carrying ARGs could be the main cause of ARG proliferation.In addition,significant co-occurrence relationships between ARGs and mobile genetic elements were also observed in the sludge samples,which may also affect the ARG diversity and abundance during the organic matter treatment in the bioreactors.Overall,these findings provide new information for better understanding the ARG occurrence and dissemination caused by organic pollutants in wastewater treatment systems.

关 键 词：Organic pollutants Antibiotic resistance genes Metagenomic sequencing Mobile genetic elements Microbial community 

分 类 号：X703[环境科学与工程—环境工程]