The exposure risks associated with pathogens and antibiotic resistance genes in bioaerosol from municipal landfill and surrounding area  

作　　者：Zhishu Liang Yun Yu Xiaolong Wang Wen Liao Guiying Li Taicheng An 

机构地区：[1]Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control,Guangdong-Hong Kong-Macao Joint Laboratory for Contaminants Exposure and Health,Institute of Environmental Health and Pollution Control,Guangdong University of Technology,Guangzhou 510006,China [2]Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control,Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control,School of Environmental Science and Engineering,Institute of Environmental Health and Pollution Control,Guangdong University of Technology,Guangzhou 510006,China

出　　处：《Journal of Environmental Sciences》2023年第7期90-103,共14页环境科学学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Nos.U1901210,41877363,42130611,and 42177410);the Science and Technology Project of Guangdong Province,China(No.2021A0505030070);the Natural Science Foundation of Guangdong Province(No.2019A1515010599);the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Z032);the Young S&T Talent Training Program of Guangdong Provincial Association,China(No.2022QNRC23).

摘　　要：Pathogenic microbes with antibiotic resistance can thrive on municipal solid waste as nutrients and be aerosolized and transported to vicinities during waste disposal processes.However,the characterization of pathogenic bioaerosols and assessment of their exposure risks are lacking.Herein,particle size,concentration,activity,antibiotic resistance,and pathogenicity of airborne microorganisms were assessed in different sectors of a typical landfill.Results showed that active sector in downwind direction has the highest bioaerosol level(1234 CFU/m3),while residential area has the highest activity(14.82 mg/L).Botanical deodorizer from mist cannon can effectively remove bioaerosol.Most bioaerosols can be inhaled into respiratory system till bronchi with sizes ranging from 2.1−3.3 and 3.3−4.7μm.Pathogenic bacteria(Bacilli,Bacillus,and Burkholderia-Paraburkholderia)and allergenic fungi(Aspergillus,Cladosporium,and Curvularia)prevailed in landfill.Although high abundance of microbial volatile organic compounds(mVOCs)producing bioaerosols were detected,these mVOCs contributed little to odor issues in landfill.Notably,surrounding areas have higher levels of antibiotic-resistance genes(ARGs)than inner landfill with tetC,acrB,acrF,mdtF,and bacA as dominant ones.Most ARGs were significantly correlated with bacterial community,while environmental parameters mainly influenced fungal prevalence.These findings can assist in reducing and preventing respiratory allergy or infection risks in occupational environments relating to waste management.

关 键 词：BIOAEROSOL Pathogenic community mVOCs Antibiotic-resistance genes Health risk Botanical deodorizer 

分 类 号：X705[环境科学与工程—环境工程] X513