A sustained-release microcarrier effectively prolongs and enhances the antibacterial activity of lysozyme  

作　　者：Mei Du Jingzhang Liu Fengbang Wang Lei Bi Chunyan Ma Maoyong Song Guibin Jiang 

机构地区：[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Science,Beijing 100085,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Environmental Sciences》2023年第7期128-138,共11页环境科学学报（英文版）

基　　金：This work was supported by the National Key R&D Program of China(No.2021YFA0910300);the National Natural Science Foundation of China(Nos.22125606 and 21976198);the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDPB2005).

摘　　要：Bacterial infections have become a great threat to public health in recent years.A primary lysozyme is a natural antimicrobial protein;however,its widespread application is limited by its instability.Here,we present a poly(N-isopropylacrylamide)hydrogel inverse opal particle(PHIOP)as a microcarrier of lysozyme to prolong and enhance the efficiency against bacteria.This PHIOP-based lysozyme(PHIOP-Lys)formulation is temperature-responsive and exhibits long-term sustained release of lysozyme for up to 16 days.It shows a potent antibacterial effect toward both Escherichia coli and Staphylococcus aureus,which is even higher than that of free lysozyme in solution at the same concentration.PHIOPs-Lys were demonstrated to effectively inhibit bacterial infections and enhance wound healing in a full-thickness skin wound rat model.This study provides a novel pathway for prolonging the enzymatic activity and antibacterial effects of lysozyme.

关 键 词：Bacterial infection LYSOZYME TEMPERATURE-RESPONSIVE Inverse opal particle Wound healing 

分 类 号：R978.1[医药卫生—药品]