白念珠菌加剧酒精性肝病炎症损伤的潜在机制研究  

Potential mechanism of Candida albicans exacerbating inflammatory injury in alcoholic liver disease

作　　者：肖楠 吴嘉迪 卞海 颜贵明 XIAO Nan;WU Jiadi;BIAN Hai;YAN Guiming(College of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]华中科技大学基础医学院,湖北武汉430074

出　　处：《皖南医学院学报》2023年第2期116-120,共5页Journal of Wannan Medical College

基　　金：安徽省自然科学基金项目(2008085MH300)。

摘　　要：目的:研究白念珠菌(Candida albicans)对酒精性肝病(ALD)炎症损伤的影响。方法:构建ALD小鼠模型,将30只小鼠随机分为正常对照组、ALD模型组和ALD+C.albicans干预模型组,检测小鼠血清生化甘油三酯(TG)和总胆固醇(T-CHO)水平,肝脏HE染色观察病理学变化并评估脂肪变性分度,ELISA法检测血清肿瘤坏死因子α(TNF-α)和干扰素-γ(IFN-γ)水平,RT-PCR和qRT-PCR检测肝脏tnf-α、dectin-1和白细胞介素-1β(il-1β)基因表达水平,Western blot检测肝脏Dectin-1蛋白表达水平。结果:ALD+C.albicans干预模型组肝脏HE染色可见大量脂肪空泡,脂肪变性分度升高(P<0.05),血清TG、T-CHO、TNF-α和IFN-γ水平升高(P<0.05),肝脏tnf-α、dectin-1和il-1βmRNA和Dectin-1蛋白表达升高(P<0.05)。结论:本研究结果表明C.albicans可能通过升高Dectin-1/IL-1β信号通路表达并释放TNF-α与IFN-γ,加剧ALD炎症损伤。Objective:To study the effect of Candida albicans(C.albicans)on inflammatory damage in alcoholic liver disease(ALD).Methods:Mouse models of ALD were initially constructed,and then 30 mice were randomized to control group,ALD model group and ALD+C.albicans intervention model group.Serum biochemical triglyceride(TG)and total cholesterol(T-CHO)levels were measured.Liver tissues were stained by H&E to observe the pathological changes,and evaluate the grading of steatosis.ELISA was used to detect serum levels of TNF-αand IFN-γ.RT-PCR and qRT-PCR were performed to detect liver tnf-α,dectin-1 and interleukin-1β(il-1β)gene levels.Western blot was used to detect the expression of Dectin-1 protein in the liver tissues.Results:In the ALD+C.albicans intervention model group,liver tissues by H&E staining showed a large number of fat vacuoles,and the grading of steatosis was significantly increased(P<0.05).Serum TG,T-CHO,TNF-αand IFN-γlevels were significantly increased(P<0.05),and tnf-α,dectin-1 and il-1βmRNA and Dectin-1 protein were highly expressed in the liver(P<0.05).Conclusion:The results of this study suggest that C.albicans may exacerbate inflammatory damage in ALD by increasing the expression of Dectin-1/IL-1βsignaling pathways and releasing TNF-αand IFN-γ.

关 键 词：白念珠菌 酒精性肝病 Dectin-1/IL-1β信号通路 

分 类 号：R512.6[医药卫生—内科学] R575.5[医药卫生—临床医学] R-332