西多福韦促进2型重组腺相关病毒体外转导分析  

In Vitro Transduction Analysis of Recombinant Adeno-AssociatedVirus Type 2 Promoted by Cidofovir

作　　者：肖桂清 刁勇[2] 黄晓平 段训威 白如玉[2] XIAO Guiqing;DIAO Yong;HUANG Xiaoping;DUAN Xunwei;BAI Ruyu(College of Light Industry,Liming Vocational University,Quanzhou Fujian 362000,China;School of Medicine,Huaqiao University,Quanzhou Fujian 362021,China;School of Chemical Engineering and Materials,Quanzhou Normal University,Quanzhou Fujian 362000,China;School of Oceanology and Food Science,Quanzhou Normal University,Quanzhou Fujian 362000,China)

机构地区：[1]黎明职业大学轻工学院,福建泉州362000 [2]华侨大学医学院,福建泉州362021 [3]泉州师范学院化工与材料学院,福建泉州362000 [4]泉州师范学院海洋与食品学院,福建泉州362000

出　　处：《泉州师范学院学报》2023年第2期35-40,共6页Journal of Quanzhou Normal University

基　　金：国家自然科学基金资助项目(81502687);福建省中青年教师教育科研项目(JAT210888);黎明职业大学规划项目(LZ202215)。

摘　　要：为研究小分子药物西多福韦(CDV)对2型重组腺相关病毒(rAAV2)体外转导效率的影响,采用不同剂量CDV干预rAAV2-GFP和rAAV2-LacZ载体转导Hela细胞,从转基因表达量、阳性细胞数量、胞内病毒基因组数等方面研究CDV对rAAV2转导的影响.结果表明,CDV能促进rAAV2体外转导Hela细胞,转基因表达量、阳性细胞数、胞内病毒基因组数与对照组相比,差异具有统计学意义,且其促进作用呈现一定的剂量和时间依赖性.To reveal the effects of small molecule drug Cidofovir(CDV)in the transduction of the recombinant adeno-associated virus type 2(rAAV2).Different doses of CDV were used to intervene rAAV2 vector transduction of different reporter genes(GFP and LacZ)in Hela cells.The effect of CDV on rAAV2 transduction was studied from the aspects of transgene expression,positive cell number and gene number in Hela cells.The experimental results showed that CDV could promote rAAV2 transduction,and the differences in transgene expression,positive cell number and genome number were statistically significant compared with those in the control ground.Furthermore,the time-dosage dependent relationship existed in the transduction efficiency of rAAV2 in Hela cells.

关 键 词：2型重组腺相关病毒 西多福韦 转导效率 增效剂 

分 类 号：R511.8[医药卫生—内科学] R915[医药卫生—临床医学]