SHMT2结合并上调SQSTM1促进结肠癌细胞增殖  

SHMT2 Promotes the Proliferation of Colon Cancer Cells Through Binding to SQSTM1 and Up-regulating its Expression

作　　者：陈前智 沈娜 李雪芹 CHEN Qianzhi;SHEN Na;LI Xueqin(Department of Breast and Thyroid Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)

机构地区：[1]华中科技大学同济医学院附属协和医院乳腺甲状腺外科,武汉430022

出　　处：《医药导报》2023年第6期814-818,共5页Herald of Medicine

基　　金：湖北省自然科学基金资助项目(2021-CFB108)。

摘　　要：目的探索SHMT2调控结肠癌细胞增殖的分子机制。方法通过免疫组化染色及生物信息分析来验证SHMT2在结肠癌组织中的表达和作用,通过细胞克隆形成实验检测SHMT2对结肠癌细胞增殖能力的影响,利用免疫共沉淀法、质粒转染、蛋白印迹实验等验证SHMT2在结肠癌中的作用机制。结果免疫组化染色揭示SHMT2蛋白在结肠癌肿瘤组织中显著高表达,通过生信分析发现SHMT2 mRNA水平与结肠癌的不良预后相关,在细胞实验中发现SHMT2低表达或者SHMT2抑制剂可显著抑制结肠癌细胞增殖,其作用机制可能是SHMT2通过与SQSTM1结合并上调其蛋白水平来促进结肠癌细胞增殖。结论该研究发现SHMT2-SQSTM1信号轴对结肠癌细胞增殖的关键调控作用,为结肠癌相关分子机制研究提供了新的线索和方向。Objective To explore the molecular mechanism of SHMT2 regulating the proliferation of colon cancer cells.Methods This study verified the expression and function of SHMT2 in colon cancer through immunohistochemical staining and bioinformatics analysis,detected the changes of colon cancer cell proliferation ability through colony formation experiments,and determined the protein regulatory mechanism of action through methods such as immunoprecipitation,plasmid transfection,and protein blotting.Results Compared with normal colon tissues,SHMT2 was significantly overexpressed in colon cancer tumor tissue.SHMT2 was related to poor prognosis of colon cancer.In cell experiments,it was found that SHMT2 knockdown or SHMT2 inhibitor could significantly inhibit the proliferation of colon cancer cells.The molecular mechanism was that SHMT2 promoted colon cancer cell proliferation by binding to SQSTM1 and up-regulating its protein level.Conclusion This study discovered the key regulatory role of the SHMT2-SQSTM1 signaling axis in colon cancer cell proliferation,provided new clues and directions for the study of colon cancer-related molecular mechanisms.

关 键 词：结肠癌 丝氨酸羟甲基转移酶2(SHMT2) SHMT2抑制剂 SQSTM1 增殖 

分 类 号：R966[医药卫生—药理学] R735.3[医药卫生—药学]