山地虎耳草质量控制方法研究  

Quality Control of Saxifraga montana

作　　者：苏媛 刘安平 刘学良 李维业 刘海青 石春兰 问静 王永晶 牛贵姗 王开祥 韩达斌 杨增亮 祁彦凯 SU Yuan;LIU Anping;LIU Xueliang;LI Weiye;LIU Haiqing;SHI Chunlan;WEN Jing;WANG Yongjing;NIU Guishan;WANG Kaixiang;HAN Dabin;YANG Zengliang;QI Yankai(Xining Center for Food and Drug Inspection&Testing,Xining 810007,China;Qinghai Drug Evaluation and Verification Center,Xining 810007,China)

机构地区：[1]西宁市食品药品检验检测中心,西宁810007 [2]青海省药品审评核查中心,西宁810007

出　　处：《医药导报》2023年第6期883-887,共5页Herald of Medicine

基　　金：青海省基础研究项目(2020-ZJ747)。

摘　　要：目的建立山地虎耳草的质量控制方法。方法采用薄层色谱法对山地虎耳草中金丝桃苷进行定性鉴别;采用高效液相色谱法测定山地虎耳草中金丝桃苷和当药醇苷含量。结果金丝桃苷薄层色谱鉴别斑点清晰,特征明显,专属性强,可行性良好,12批样品均显示有金丝桃苷的斑点;高效液相色谱法测定金丝桃苷和当药醇苷2种成分分别在0.1246~1.4946μg(r=0.9999)、0.0453~0.5436μg(r=0.9999)范围内与其峰面积积分值均呈良好的线性关系。结论该文建立的质量控制方法简便、准确、可行,可用于山地虎耳草的质量控制。Objective To establish the method of quality control for Saxifraga montana Methods Hypericin in Saxifraga montana was identified by thin layer chromatography(TLC).Hypericin and Swertianolin in Saxifraga montana were determined by high performance liquid chromatography(HPLC).Results TLC identification for Hypericin features clear identification spots,strong specificity,and good feasibility,12 batches of samples show a spot of ypericin.Hypericin and Swertianolin content had a good linearity in the range of 0.1246-1.4946μg(r=9999)and 0.0453-0.5436μg(r=0.9999).Conclusion The method is simple,accurate,feasible,and can be used for quality control of Saxifraga montana.

关 键 词：山地虎耳草 金丝桃苷 当药醇苷 薄层 色谱法 高效液相 色谱法 

分 类 号：R29[医药卫生—民族医学] R927[医药卫生—中医学]