灌服酒炙车前子活性组分的胎衣不下奶牛血清代谢产物LC-MS/MS法分析  

Identification of in Vivo Metabolites of Active Components of Semen Plantaginis by Stir-frying with Wine on Cure of Cows with Retained Placenta by LC-MS/MS

作　　者：鲁文赓[1] 周继伟 田春雨 司琳清 袁思琦 谢何昕 申贵男 Lu Wengeng;Zhou Jiwei;Tian Chunyu;Si Linqing;Yuan Siqi;Xie Hexin;Shen Guinan(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing163319;Yushu City Agricultural Comprehensive Administrative Law-enforcement Unit;College of Life Science and Technology,Heilongjiang Bayi Agricultural University)

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]吉林省榆树市农业综合行政执法大队 [3]黑龙江八一农垦大学生命科学技术学院

出　　处：《黑龙江八一农垦大学学报》2023年第2期29-36,136,共9页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省自然科学基金联合引导项目(LH2020C085);现代农业(奶牛)产业技术体系建设专项资金资助(CARS-36)。

摘　　要：早期研究过程中发现活性组分Ⅲ和Ⅵ为治疗奶牛胎衣不下的有效物质,并已阐明其化学成分,但尚未鉴定活性组分在体内的代谢产物,因此研究旨在利用液相色谱-串联质谱(LC-MS/MS)对活性组分Ⅲ和Ⅵ的血清样品进行分析。以ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm)为色谱柱条件,流动相以水(含0.05%甲酸)-乙腈进行梯度洗脱,采用ESI电喷雾离子源,正、负离子模式下收集活性组分Ⅲ和Ⅵ的血清样品的质谱数据,并根据碎片离子以及相对分子质量等信息与网络数据库以及相关参考文献进行比对。活性组分Ⅲ发现包括胺类、脂类在内的共24个化合物,活性组分Ⅵ包括酚类、烷类等共26个化合物,为研制高效、经济和便捷的防治奶牛胎衣不下的中药针剂提供了新方向。The previous research had determined that the effective substances of semen plantaginis by stir-frying with wine on cure of cows with retained placenta were active components III and VI,and defined the chemical components of active components,but the metabolites in vivo of active components had not been identified.Therefore,the serum samples components III and VI was identified by LC-MS/MS.ACQUITY UPLC HSS T3(2.1 mm x 100 mm,1.8μm)was used as chromatographic column,water(containing 0.05%formic acid)-acetonitrile was used as mobile phase for gradient elution and ESI source was used,mass spectrum data were acquired in positive and anion ion mode,and was compared with database and relevant literature according to the accurate charge-mass ratio and the MS/MS data information.Twenty-four chemical components were found in active components III,mainly including amines,lipids.Twenty-six compounds were identified in active component VI,including phenols and alkanes.The results laid a practical foundation for the development of high efficiency,economic and convenient Chinese medicine injection for preventing and controlling the retained placenta in the dairy cows.

关 键 词：酒炙车前子 胎衣不下 液相色谱-串联质谱 代谢产物 奶牛 

分 类 号：S853.7[农业科学—临床兽医学] S857.2[农业科学—兽医学]