硫酸头孢喹肟乳房注入剂中头孢喹肟含量HPLC通用性测定方法的建立  

Establishment of Universal HPLC Method for the Determination of Cefquinome in Cefquinome Sulfate Intramammary Infusion

作　　者：韩宁宁[1] 彭文绣 王轩 赵晖[1] 杨秀玉[1] 戴青[1] 赵富华[1] HAN Ning-ning;PENG Wen-xiu;WANG Xuan;ZHAO Hui;YANG Xiu-yu;DAI Qing;ZHAO Fu-hua(China Institute of Veterinary Drug Control,Beijing 100081,China)

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国兽药杂志》2023年第5期33-40,共8页Chinese Journal of Veterinary Drug

基　　金：农业行业(国家)标准项目兽药国家标准制修订计划(编号:2017H002)。

摘　　要：建立了适用于国内硫酸头孢喹肟乳房注入剂(泌乳期和干乳期)中头孢喹肟含量测定的HPLC通用性方法。采用与《中国兽药典》硫酸头孢喹肟原料含量测定一致的高效色谱条件,使用有机溶剂破乳后加入流动相超声的提取方法,保证了方法的一致性和适用性。方法验证结果显示,本方法专属性较强,准确度较高,重复性和耐用性良好,适用于对国内硫酸头孢喹肟乳房注入剂的含量测定,被收载入2020年版《中国兽药典》。A universal method for the determination of cefquinome sulfate intramammary infusion(lactating cow and dry cow)was established.The chromatographic conditions consistent with the content determination of cefquinome sulfate raw material in Chinese Veterinary Pharmacopoeia,and the extraction method of adding mobile phase to ultrasound after demulsification with organic solvent ensured the consistency and applicability of the method.The validation results of the method showed that the specificity,accuracy,repeatability and durability of this method were strong,and it was suitable for the content determination of domestic cefquinome sulfate intramammary infusion.It was included in the Chinese Veterinary Pharmacopoeia(2020).

关 键 词：硫酸头孢喹肟乳房注入剂(泌乳期和干乳期) 含量测定 通用性 破乳 

分 类 号：S859.79[农业科学—临床兽医学]