‘潢川金桂’花瓣衰老过程中细胞程序性死亡的生理机制  

Programmed cell death events during the petal senescence of Osmanthus fragrans‘Huangchuan Jingui’

作　　者：贺贝 蔡璇 曾祥玲[1] 王彩云[2] 周媛 邹晶晶[1] HE Bei;CAI Xuan;ZENG Xiangling;WANG Caiyun;ZHOU Yuan;ZOU Jingjing(National Forestry and Grassland Administration Engineering Research Center for Osmanthus fragrans,Hubei University of Science and Technology,Xianning 437100,Hubei,China;Key Laboratory for Biology of Horticultural Plants,Ministry of Education,Huazhong Agricultural University,Wuhan 430070,Hubei,China;Wuhan Academy of Agricultural Sciences,Wuhan 430070,Hubei,China)

机构地区：[1]湖北科技学院国家林业草原桂花工程技术研究中心,湖北咸宁437100 [2]华中农业大学园艺植物生物学教育部重点实验室,湖北武汉430070 [3]武汉市农业科学院林业果树研究所,湖北武汉430070

出　　处：《浙江农林大学学报》2023年第3期475-480,共6页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(32271951,32101581,31700617);湖北省科技创新专项(2021BBA098);湖北省中央引导地方专项(2022BEG263)。

摘　　要：【目的】探索了结实型桂花Osmanthus fragrans花瓣衰老过程中,生理生化变化,为提高桂花园林赏花价值与经济用花价值提供理论依据。【方法】以‘潢川金桂’O.fragrans‘Huangchuan Jingui’为材料,采用蛋白质印迹法测定细胞质中细胞色素c的释放,高效液相色谱法检测腺嘌呤核苷三磷酸(ATP),气相色谱法测定内源乙烯释放,流式细胞法检测核DNA变化,以及检测桂花开放及衰老过程中超氧自由基等生理指标变化。【结果】①桂花花瓣中超氧阴离子和过氧化氢质量摩尔浓度在初花期达到最高值,随后逐渐下降。②细胞色素c的含量在初花期开始积累至盛花期最高,在盛花末期开始减少至消失,而ATP质量分数一直呈下降趋势。③乙烯释放量从盛花末期开始逐渐上升至萎蔫期达到最大值,此时花瓣中的核物质也基本消失。④桂花花瓣中活性氧的激发、细胞色素c释放和ATP持续下降明显发生于初花期,为早期细胞程序性死亡(PCD)事件;乙烯跃变、DNA降解发生在盛花末期之后,为晚期PCD事件。【结论】线粒体功能活性下降可能是桂花花瓣早期PCD信号,内源乙烯跃变引起花瓣萎蔫失水和DNA降解可能是PCD执行的结果。图5参31。[Objective]This study,with an investigation of the physiological and biochemical changes during the senescence of sweet Osmanthus fragrans flowers,is aimed to provide guidance for improving both ornamental and economical value of O.fragrans.[Method]With O.fragrans‘Huangchuan Jingui’selected as the experimental material,the release of cytochrome c(Cyt c)in the cytoplasm was determined by Western blotting;the content of adenosine triphosphate(ATP)was determined by high performance liquid chromatography;the endogenous ethylene production was determined by gas chromatography;the content of nuclear DNA were detected by flow cytometry and the changes of other physiological indicators such as free radicals during the flowering were also detected.[Result](1)The amount of reactive oxygen species(ROS)in osmanthus petals reached the highest value in the initial flowering stage,and then it gradually decreased;(2)The content of Cyt c began to accumulate in the initial flowering stage and reached the highest in the full flowering stage,but decreased at the late full flowering stage whereas the ATP content declined during the whole flowering stages;(3)The ethylene release gradually increased from late full flowering stage to the maximum in the wilting petals,at which time the nuclear matter in the petals also disappeared;(4)The excitation of ROS,the release of Cyt c into the cytoplasm and the continuous decrease of ATP in petals of O.fragrans‘Huangchuan Jingui’mainly occurred in the initial flowering stage,which was an early programmed cell death(PCD)event while the climacteric of ethylene production and DNA degradation occurred in the late flowering stage,referred to as late PCD events.[Conclusion]Just as the decrease of mitochondrial functional activity may be the early PCD signal during the petal senescence of sweet osmanthus,petal wilting and DNA degradation related to endogenous ethylene production may be the result of PCD execution.[Ch,5 fig.31 ref.]

关 键 词：桂花 细胞程序性死亡 花瓣衰老 细胞色素C 腺嘌呤核苷三磷酸(ATP) 乙烯 

分 类 号：S722.3[农业科学—林木遗传育种]