大蒜油微乳液的制备及抑藻性能  

Preparation and algae inhibition of garlic oil microemulsion

作　　者：黄杨 郭明[2] 王义平[1] 叶碧欢 陈友吾[3] 王为宇 HUANG Yang;GUO Ming;WANG Yiping;YE Bihuan;CHEN Youwu;WANG Weiyu(College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;College of Chemistry and Materials Engineering,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Forest Protection Research Institute,Zhejiang Academy of Forestry,Hangzhou 310023,Zhejiang,China;Hangzhou Lin’an Agricultural and Forestry Technology Promotion Center,Hangzhou 311399,Zhejiang,China)

机构地区：[1]浙江农林大学林业与生物技术学院,浙江杭州311300 [2]浙江农林大学化学与材料工程学院,浙江杭州311300 [3]浙江省林业科学研究院森林保护研究所,浙江杭州310023 [4]杭州市临安区农林技术推广中心,浙江杭州311399

出　　处：《浙江农林大学学报》2023年第3期627-635,共9页Journal of Zhejiang A&F University

基　　金：浙江省省院合作林业科技项目(2019SY09);中央财政林业科技推广项目([2021]TS16号)。

摘　　要：【目的】将大蒜油加工制备成大蒜油微乳液,以期提高大蒜油的稳定性,并开发新型植物源灭藻剂。【方法】运用相转变法和拟三元图探讨表面活性剂和助表面活性剂种类以及质量比(Km)对大蒜油微乳液体系形成的影响并进行正交实验。考察了以最优条件制备的大蒜油微乳液在不同温度、不同pH以及5 000和10 000 r·min^(-1)离心条件下的稳定性,并进行了大蒜油微乳液抑藻实验。【结果】最佳制备条件为25℃、以吐温80 (Tween 80)为表面活性剂,乙醇和正丁醇为助表面活性剂,三者质量比为1∶1∶1,混合表面活性剂与大蒜油质量比为4∶6。所得微乳液为黄色澄清透明状液体并伴有淡蓝色光泽,有效粒径为20.1 nm,多分散系数为0.144。320 mg·L^(-1)大蒜油微乳液的抑藻率为82.54%。【结论】成功制备的水包油型大蒜油微乳液,稳定性良好,粒径大小合适,抑藻效果明显,有望制备成新型的灭藻剂。图9表3参31。[Objective]This study aims to prepare microemulsion from garlic oil in order to improve the stability of garlic oil and develop a new type of plant-derived algaecide.[Method]The effects of surfactant and co-surfactant types and mass ratio(Km)on the formation of garlic oil microemulsion system were investigated by phase transition method and pseudo-ternary diagram,and orthogonal experiments were carried out.The stability of garlic oil microemulsion prepared under the optimal conditions under different temperature,pH and centrifugation conditions of 5000 and 10000 r·min^(-1) was investigated,and the algae inhibition of garlic oil microemulsion experiment was carried out.[Result]The optimum preparation conditions were 25℃,Tween 80 as surfactant,ethanol and n-butanol as co-surfactants.The mass ratio of the three was 1∶1∶1,and the mass ratio of mixed surfactant and garlic oil was 4∶6.The obtained microemulsion was yellow clear transparent liquid with light blue luster.The average particle size was 20.1 nm and the polydispersity coefficient was 0.144.The algae inhibition rate of 320 mg·L^(-1) garlic oil microemulsion was 82.54%.[Conclusion]The successfully prepared oil-in-water type garlic oil microemulsion has good stability,suitable particle size,and obvious algae inhibitory effect.It is expected to be a new algaecide.[Ch,9 fig.3 tab.31 ref.]

关 键 词：大蒜油微乳液 拟三元相图 表面活性剂 香榧绿藻 

分 类 号：S769[农业科学—森林保护学]