不同日龄湖羊羔羊瘤胃上皮细菌的差异  

Differences of ruminal epithelial bacterial diversity of Hu lambs at different ages

作　　者：纪雯雯 云燕 王翀 茅慧玲 JI Wenwen;YUN Yan;WANG Chong;MAO Huiling(College of Animal Science and Technology/College of Veterinary Medicine,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学动物科技学院/动物医学院,浙江杭州311300

出　　处：《浙江农林大学学报》2023年第3期673-679,共7页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31902179)。

摘　　要：【目的】比较不同日龄湖羊Ovis aries羔羊瘤胃上皮细菌多样性及菌群组成差异,为幼龄反刍动物早期断奶提供理论依据。【方法】选用10只出生体质量相近的初生湖羊羔羊,分别于日龄30 d (D30)和45 d (D45)各屠宰5只以获得瘤胃上皮组织,对其进行测序及微生物多样性分析。【结果】D45组羔羊瘤胃上皮细菌的操作分类单元(OTU)个数显著多于D30组羔羊(P<0.05),Chao1指数D45组有增加的趋势(P=0.07)。与D45组相比,D30组羔羊瘤胃细菌弯曲杆菌属Campylobacter和肠单胞菌Intestinimonas的相对丰度显著降低(P<0.05),拟杆菌属Bacteroides和小杆菌属Dialister的相对丰度有降低的趋势(P=0.083);而萨顿氏菌属Suttonella、脱硫弧菌属Desulfobulbus和单胞菌属Brachymonas的相对丰度显著增加(P<0.05),琥珀酸菌属Succiniclasticum有增加的趋势(P=0.083)。相关性分析发现:萨顿氏菌属与瘤胃挥发性脂肪酸含量呈正相关(P<0.05),拟杆菌属、琥珀酸菌属、瘤胃球菌属Ruminococcus和奇异菌属Atopobium与瘤胃乳头长度呈负相关(P<0.01),琥珀酸菌属与瘤胃乳头宽度呈正相关(P<0.01)。【结论】不同日龄羔羊的瘤胃上皮细菌定植存在差异,这些菌群参与瘤胃挥发性脂肪酸代谢及瘤胃乳头发育。图7表2参32。[Objective]This study,with a comparison of the diversity and composition of rumen epithelial bacteria among Hu lambs(Ovis aries)of different ages,is aimed to provide basic data for early weaning of young ruminants.[Method]Ten male Hu lambs,with similar birth weight,were used in this study.At 30 days of age(D30)and 45 days of age(D45),five lambs were randomly selected and slaughtered to obtain rumen epithelial tissues(n=5),respectively.Sequencing and microbial diversity analysis were performed on them.[Result]The Operational Taxonomic Unit(OTU)number in D45 group was larger than that in D30 group(P<0.05),with the Chao1 index in D45 group showing a tendency to increase(P=0.07).The relative abundances of Campylobacter and Intestinimonas declined(P<0.05)with age,while the relative abundances of Suttonella,Desulfobulbus and Brachymonas increased(P<0.05)with age.The relative abundances of Bacteroides and Dialister tended to decrease(P=0.083),whereas Succiniclasticum tended(P=0.083)to increase.The abundance of Suttonella was positively(P<0.05)correlated with rumen total volatile fatty acid concentration,while the abundances of Bacteroides,Succiniclasticum,Ruminococcus and Atopobium were negatively(P<0.01)correlated with ruminal papillae length,and the abundance of Succiniclasticum was positively(P<0.01)correlated with rumen papillae width.[Conclusion]It was revealed that colonization by ruminal epithelial bacteria is age related and might participate in the metabolism of volatile fatty acid and ruminal papillae development of the rumen.[Ch,7 fig.2 tab.32 ref.]

关 键 词：日龄 湖羊羔羊 瘤胃上皮 瘤胃细菌 

分 类 号：S811.6[农业科学—畜牧学]