深海芽孢杆菌Bacillus sp. LM-24对结晶紫的吸附研究  

Adsorption of Crystalline Violet by Bacillus sp.LM-24 in Deep Sea

作　　者：徐湘薇 张继福[6] 张云[1,2,3,4,7] 胡云峰 XU Xiangwei;ZHANG Jifu;ZHANG Yun;HU Yunfeng(South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou Guangdong 510301,China;CAS Key Laboratory of Tropical Marine Biological Resources and Ecology(South China Sea Institute of Oceanology,Chinese Academy of Sciences),Guangzhou Guangdong 510301,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou Guangdong 511458,China;Guangdong Key Laboratory of Marine Materia Medical(South China Sea Institute of Oceanography,Chinese Academy of Sciences),Guangzhou Guangdong 510301,China;University of Chinese Academy of Sciences,Beijing 100049,China;Guangdong Provincial Hospital of Chinese Medicine,Guangzhou Guangdong 510120,China;Equipment Public Service Center,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou Guangdong 510301,China)

机构地区：[1]中国科学院南海海洋研究所,广东广州510301 [2]中国科学院热带海洋生物资源与生态重点实验室(中国科学院南海海洋研究所),广东广州510301 [3]南方海洋科学与工程广东省实验室(广州),广东广州511458 [4]广东省海洋药物重点实验室(中国科学院南海海洋研究所),广东广州510301 [5]中国科学院大学,北京100049 [6]广东省中医院,广东广州510120 [7]中国科学院南海海洋研究所仪器设备公共服务中心,广东广州510301

出　　处：《广西师范大学学报（自然科学版）》2023年第3期221-231,共11页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(21302199);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0406)。

摘　　要：利用深海微生物中筛选出的对结晶紫具有优势吸附能力的菌株Bacillus sp. LM-24从废水中分离结晶紫。采用单因素实验优化其反应条件,选用无机载体硅胶和有机载体树脂对其进行固定,分析无机盐对吸附作用的影响及其吸附动力学与吸附等温线。结果表明游离菌株在最适吸附剂浓度1.216 g/L、pH=9.0、温度30℃时,反应10 min对30 mg/L结晶紫去除率可达86%;在不同pH和浓度时,固定化微生物吸附作用较游离微生物更加稳定。动力学测定显示3种吸附剂均存在物理和化学吸附,等温线测定显示游离菌株和硅胶固定化菌株与Langmuir模型拟合度更高,而大孔树脂固定化菌株与Freundlich拟合度更高。Bacillus sp. LM-24对结晶紫具有快速且高效的吸附作用,且固定化技术能有效提高其对外界环境的稳定性,可作为结晶紫染料脱色的潜力开发菌株。The crystalline violet was isolated from wastewater by using Bacillus sp.LM-24,a strain selected from deep-sea microorganisms with a dominant adsorption capacity for crystalline violet.The reaction conditions were optimized by single-factor experiments,and the inorganic carrier silica gel and organic carrier resin were selected for its immobilization.The effects of inorganic salts on the adsorption and its adsorption kinetics and adsorption isotherms were analyzed.The results showed that the adsorption of 30 mg/L crystalline violet was 86%removed by the free strain at the optimum adsorbent concentration of 1.216 g/L,optimum pH 9.0 and 30℃for 10 min,the adsorption of immobilized microorganisms was more stable than that of free microorganisms at different pH and concentration.Kinetic measurements showed that all three adsorbents were physically and chemically adsorbed,and isotherm measurements showed that the free and silica immobilized strains fit the Langmuir model better,while the macroporous resin immobilized strains fit the Freundlich model better.The immobilization technique could improve its stability to the external environment,and it could be a potential strain for the decolorization of crystalline violet dye.

关 键 词：深海微生物 芽孢杆菌 吸附 结晶紫 固定化微生物 

分 类 号：X703[环境科学与工程—环境工程]