山羊鼻内肿瘤病毒广东株的全基因测序与遗传进化分析  

Whole gene sequencing and genetic evolutionary analysis of a Cantonese strain of goat endonasal tumor virus

作　　者：张浩权 郭亚晶 韩慧 莫美连 刘明杰 黄良宗 ZHANG Hao-quan;GUO Ya-jing;HAN Hui;MO Mei-lian;LIU Ming-Jie;HUANG Liang-zong(School of Life Science and Engineering,Foshan University,Foshan 528225,China;Guangdong Findergene Technology Co.,Ltd.,Foshan 528000,China)

机构地区：[1]佛山科学技术学院生命科学与工程学院,广东佛山528225 [2]广东方道基因生物科技有限公司,广东佛山528000

出　　处：《佛山科学技术学院学报（自然科学版）》2023年第3期52-58,共7页Journal of Foshan University(Natural Science Edition)

基　　金：广东省现代草牧业(羊)产业技术体系(2019KJ127);广东省企业科技特派员项目(Kh21368)。

摘　　要：为了解山羊地方性鼻内肿瘤病毒(ENTV-2)的基因组特征和遗传变异情况,通过RT-PCR及序列分析方法从广东省某羊场送检病羊组织中鉴定出一株ENTV-2,将其命名为GDZJ2022。全基因组测序结果分析显示,GDZJ2022与GenBank中26株ENTV参考毒株核苷酸序列相似性为86.5%~96.9%,其中与四川ENTV-2-CHN3毒株序列同源性最高(96.9%),并处于同一分支。env核苷酸序列分析结果显示,GDZJ2022与四川株ENTV-2-CHN1同源性最高(98.6%);gag核苷酸序列分析结果显示,GDZJ2022与GDQY-2017同源性最高(95.2%)。In order to understand the genomic characteristics and genetic variation of enzootic nasal tumor virus(ENTV-2),in this study,a strain of ENTV-2 was identified from diseased goat tissues in Guangdong Province by RT-PCR and sequence analysis,and named GDZJ2022.Whole-genome sequencing results showed that GDZJ2022 had 86.5%-96.9% nucleotide sequence similarity with the 26 reference strains of ENTV in GenBank,with the highest sequence homology(96.9%)with the Sichuan ENTV-2-CHN3 strain and being in the same branch.The env nucleotide sequence analysis showed that GDZJ2022 had the highest sequence homology(98.6%)with the Sichuan strain.The gag nucleotide sequence analysis showed that GDZJ2022 had the highest homology(95.2%)with GDQY-2017.

关 键 词：山羊 地方性鼻内肿瘤病毒 ENV GAG 全基因组测序 

分 类 号：S858.27[农业科学—临床兽医学]