茉莉酸信号关键转录因子OsMYC2影响水稻愈伤诱导和分化的功能初探  

OsMYC2,a key transcription factor in jasmonic acid signaling pathway,regulates the induction and differentiation of embryogenic callus in rice

作　　者：蒋莹莹 张华[2] 雷志伟 徐恒[2] 张恒 朱英[2] JIANG Yingying;ZHANG Hua;LEI Zhiwei;XU Heng;ZHANG Heng;ZHU Ying(College of Chemistry and Life Science,Zhejiang Normal University,Jinhua 321004,Zhejiang,China;State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products,Research Center for Crop Molecular Breeding at Zhejiang Province,Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Advanced Agricultural Sciences,Zhejiang A&F University,Hangzhou 311300,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省农业科学院病毒学与生物技术研究所,农产品质量安全危害因子与风险防控省部共建国家重点实验室,浙江省作物分子育种工程技术研究中心,浙江杭州310021 [3]浙江农林大学现代农学院,浙江杭州311300

出　　处：《浙江农业学报》2023年第5期973-982,共10页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31871226)。

摘　　要：为了了解茉莉酸(jasmonic acid,JA)信号途径基因在水稻愈伤组织诱导和分化过程中的作用,本研究对转录因子基因OsMYC 2在水稻再生过程中的功能进行了研究。利用qRT-PCR技术对OsMYC 2基因在水稻愈伤诱导和分化过程中的表达进行动态分析,同时对osmyc 2基因编辑突变体在水稻组织培养再生过程中的表型进行鉴定,并对osmyc 2突变体愈伤中的一些重要标记基因的表达进行调查和分析。qRT-PCR基因表达鉴定结果显示,OsMYC 2基因在水稻成熟胚愈伤组织诱导和分化过程中均存在一定量的表达,且在分化期的表达量相对较高。而osmyc2突变体的愈伤诱导效率和分化效率均显著低于野生型对照日本晴。同时,基因表达分析发现,OsERF 101、OsBBM 2和OsWUS等与再生相关的重要基因在osmyc 2突变体愈伤中的表达与野生型对照相比均显著下降。由此表明,OsMYC 2基因可能通过茉莉酸信号途径影响水稻成熟胚愈伤组织的诱导形成和胚性愈伤的分化。该研究对OsMYC 2在水稻再生过程中的作用进行了探索性研究,为未来深入研究茉莉酸信号途径影响水稻组织培养的分子机制提供了一定的理论基础。Functional analysis of OsMYC 2 in rice regeneration could help to reveal that the role of jasmonic acid(JA)and its signal pathway gene in the induction and differentiation of embryogenic callus.By using the qRT-PCR technology,dynamic analysis of OsMYC 2 expression was carried out during the regeneration process of rice callus.Several osmyc2 mutants generated from CRISPR/Cas9 technology,were used to study the biological function of OsMYC 2 in rice regeneration.Some marker genes,such as OsERF101,OsBBM2,OsWUS,which might play an essential role in the embryogenic callus formation,was investigated in the callus from osmyc 2 mutants and wild type rice Nipponbare.Dynamic analysis showed that OsMYC 2 was expressed in the entire rice regeneration stage,including the embryogenic callus formation and somatic embryogenesis.Expression of OsMYC 2 in callus cultured in the differentiation medium was higher than that in the induction medium.Loss of function of OsMYC 2 leads to much lower efficiency of embryogenic callus formation and somatic embryogenesis.And lower expression of OsERF101,OsBBM 2 and OsWUS in the osmyc2 mutant callus compared with the wild type suggested that OsMYC 2 regulates embryogenic callus formation and somatic embryogenesis of rice might through the JA signal pathway.These results would provide a theoretical basis for further study on the molecular mechanisms of JA signaling pathway involved in regulation of rice regeneration efficiency.

关 键 词：OsMYC2 水稻再生 基因编辑 愈伤分化 茉莉酸信号途径 

分 类 号：S511[农业科学—作物学]