RNA干扰PhCatC 1/2基因对波纹龙虾相关免疫基因表达的影响  

Effect of RNA interference with PhCatC1/2 gene on expression of related immune genes in Panulirus homarus

作　　者：梁妃爽 梁华芳[1] 黄佳宇 王潘妹 温崇庆[1] LIANG Feishuang;LIANG Huafang;Huang Jiayu;WANG Panmei;WEN Chongqing(Fisheries College,Guangdong Ocean University,Zhanjiang 524088,Guangdong,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088

出　　处：《浙江农业学报》2023年第5期1037-1047,共11页Acta Agriculturae Zhejiangensis

基　　金：广东省科技厅农业攻关项目(2013B020308009);广东海洋大学科研项目(C16394)。

摘　　要：初步研究RNA干扰PhCatC 1/2基因对波纹龙虾(Panulirus homarus)相关免疫基因表达的影响,采用qPCR技术检测PhCatC 1/2、PhTlr 13、PhLys和Phβ-G基因的表达,并观察肝胰腺组织的变化情况。结果表明:注射PhCatC 1-dsRNA后,PhCatC2表达量比对照组显著下降(P<0.05),与对照组相比PhTlr 13基因表达量在前期呈现显著升高,后下降的趋势,PhLys和Phβ-G基因的表达量均随dsRNA干扰时效的增加而增加;沉默PhCatC 2基因后PhTlr 13和Phβ-G基因的表达量随dsRNA干扰时效的增加而增加,PhCatC 1基因表达量呈现下上调交替的趋势,PhLys基因表达量表现出先下调后上调的趋势。通过PhCatC 1/2基因沉默后的分析初步推测,波纹龙虾可能通过促进Toll样受体和溶酶体通路来参与免疫应答,得出PhCatC 1/2基因具有免疫功能。Preliminary study of the effect of RNA interference with the PhCatC 1/2 gene on the expression of immune genes associated with Panulirus homarus,the expression of PhCatC1/2,PhTlr13,PhLys and Phβ-G genes were detected by qPCR and changes in hepatopancreatic tissue were observed.The results showed that the expression of PhCatC 2 was significantly different from the control group after PhCatC 1-dsRNA injection(P<0.05),the PhTlr13 gene expression showed a significant increase in the first period and then a decrease compared with the control group,expression of both PhLys and Phβ-G genes increased with increasing duration of dsRNA interference.Expression of PhTlr 13 and Phβ-G genes increased with increasing duration of dsRNA interference after silencing of the PhCatC 2 gene,PhCatC 1 gene expression showed an alternating trend of down-and up-regulation,PhLys gene expression showed a trend of down-regulation followed by up-regulation.Preliminary speculation from analysis of the PhCatC 1/2 gene after silencing that the lobster Panulirus homarus may be involved in the immune response by promoting Toll-like receptor and lysosomal pathways and concluding that the PhCatC1/2 gene has an immune function.

关 键 词：波纹龙虾 RNA干扰 PhCatC 1/2 PhTlr 13 PhLys Phβ-G 表达分析 

分 类 号：S917[农业科学—水产科学]