萝卜胞质雄性不育(Ogura CMS)芥菜材料的创制  

Creation of Ogura cytoplasmic male sterility(Ogura CMS)material of Brassica juncea

作　　者：李崇娟 吕凤仙 杨鼎 张丽琴[2,3] 兰梅 杨红丽[2,3] 徐学忠 胡靖锋[2,3] 申时品 吴毓飞[4] 和江明 董相书[1] LI Chongjuan;LYU Fengxian;YANG Ding;ZHANG Liqin;LAN Mei;YANG Hongli;XU Xuezhong;HU Jingfeng;SHEN Shipin;WU Yufei;HE Jiangming;DONG Xiangshu(Resource Plant Research Institute,Yunnan University,Kunming 650504,China;Horticulture Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Yunnan Branch of National Vegetable Improvement Center,Kunming 650205,China;Agricultural Science and Technology Promotion Center of Zhenxiong County,Zhenxiong 657200,Yunnan,China)

机构地区：[1]云南大学资源植物研究院,云南昆明650504 [2]云南省农业科学院园艺作物研究所,云南昆明650205 [3]国家蔬菜改良分中心云南分中心,云南昆明650205 [4]镇雄县农业科技推广中心,云南镇雄657200

出　　处：《浙江农业学报》2023年第5期1058-1068,共11页Acta Agriculturae Zhejiangensis

基　　金：国家大宗蔬菜产业技术体系(CARS-23-G33);云南省“万人计划”产业技术领军人才(YNWR-CYJS-2018-040);云南种子种业联合实验室项目(202205AR070001)。

摘　　要：为获得萝卜胞质雄性不育芥菜,利用远缘杂交将芥菜的B基因组导入白菜,通过连续回交法结合胚挽救技术,创制出保留白菜雄性不育基因的芥菜。用萝卜胞质雄性不育白菜CCR17001与可育叶用芥菜JC17000杂交,并以叶用芥菜为轮回亲本连续进行5代回交,获得萝卜胞质雄性不育芥菜材料,对其进行形态学、细胞学、分子生物学鉴定。结果表明:该材料植株形态与轮回亲本相似,蜜腺发育良好,雄蕊高度退化成花瓣,不育度和不育株率均为100%,且不育性稳定,有正常结籽能力,可作为芥菜优势育种的可靠材料。In order to obtain Ogura cytoplasmic male sterility Brassica juncea,the B genome of Brassica juncea was introduced into Chinese cabbage by hybridization,and the male sterility gene of Chinese cabbage was created by backcrossing combined with embryo rescue technique.With CCR17001 Ogura cytoplasmic male sterility Chinese cabbage and leaf mustard JC17000 hybridization,and leaf mustard as recurrent parent for 5 consecutive backcross generations,the Ogura cytopathic male sterile mustard material was obtained and identified by morphology,cytology and molecular biology.The results showed that the plant morphology of this material was similar to that of its recurrent parent,with well-developed nectaries,highly degenerated stamens into petals,100%sterility and sterility rate,stable sterility,normal seed setting ability,which could be used as a reliable material for breeding advantage of mustard.

关 键 词：芥菜 远缘杂交 萝卜胞质雄性不育 胚挽救 

分 类 号：S637.2[农业科学—蔬菜学]