腐皮镰刀菌侵染甘薯的转录组分析  

Transcriptomic analysis of Fusarium solani infecting sweetpotato

作　　者：罗勤川 唐伟 马居奎 陈晶伟 杨冬静[1] 高方园 孙厚俊[1] 谢逸萍[1] 张成玲[1] LUO Qinchuan;TANG Wei;MA Jukui;CHEN Jingwei;YANG Dongjing;GAO Fangyuan;SUN Houjun;XIE Yiping;ZHANG Chengling(Key laboratory of Biology and Genetic Improvement of Sweetpotato,Ministry of Agriculture and Rural Affairs,Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai Area,Sweet Potato Research Institute of Chinese Academy of Agricultural Sciences Xuzhou 221131,Jiangsu,China)

机构地区：[1]中国农业科学院甘薯研究所,江苏徐淮地区徐州农业科学研究所,农业农村部甘薯生物学与遗传育种重点实验室,江苏徐州221131

出　　处：《浙江农业学报》2023年第5期1097-1107,共11页Acta Agriculturae Zhejiangensis

基　　金：国家甘薯产业技术体系(CARS-10-GW17);国家重点研发计划(2018YFD1000703,2018YFD1000700)。

摘　　要：腐皮镰刀菌(Fusarium solani)是甘薯上的重要病原菌,易引起甘薯根腐病、腐烂溃疡病等。为明确F.solani在侵染甘薯时病原基因变化趋势,对腐烂溃疡病病菌侵染甘薯不同时期取样,通过Illumina Hiseq 4000高通量测序平台测定及分析序列。结果表明,共获得1068861644条有效数据,F.solani侵染后6 h、24 h、3 d、5 d分别筛选出了1056、995、737、935个显著差异表达基因,其中上调基因分别有587、679、430、551个,下调基因分别有469、316、307、384个,GO功能分析差异基因富集在分子功能和细胞组分,KEGG通路富集分析属于代谢和遗传信息过程。其中差异表达倍数2倍以上,且表达量水平前20的差异基因,有15个基因参与碳水化合物、能量、核苷酸以及氨基酸代谢;3个基因参与翻译和蛋白质加工和修饰等遗传信息处理;2个基因参与细胞自噬过程。随机筛选9个差异表达基因进行实时荧光定量PCR验证,结果表明,这9个基因的表达模式与转录组数据分析结果基本一致。本研究结果发现,F.solani在侵染甘薯过程中代谢活跃,这可能是其在侵染甘薯过程中需破除寄主防御并在寄主中定殖,需要自身和寄主提供大量的营养物质并转化为能量以及中间产物等物质所致。Fusarium solani is an important pathogen that causes root rot and canker disease of sweetpotato.To determine the change trend of F.solani genes during different infection stage,the high-throughput sequencing platform,Illumina Hiseq 4000 was used.The results showed that,a total of 1068861644 clean reads were obtained.Total 1056,995,737 and 935 differentially expressed genes were screened out at 6 h,24 h,3 d and 5 d after infection respectively,including 587,679,430 and 551 up-regulated genes and 469,316,307 and 384 down-regulated genes,respectively.These genes mainly belong to molecular function and cell component by GO enrichment analysis,and metabolic and genetic information processes by KEGG pathway analysis.The top 20 genes of expression level in the genes whose differential expression fold more than two times were selected to carry out further analysis.Among them,15 genes were related to metabolism,involving carbohydrate metabolism,energy metabolism,nucleotide metabolism and amino acid metabolism,3 genes were related to translation,protein processing and modification,and 2 genes were related to autophagy.Nine genes were randomly selected to verify the expression trend by quantitative real-time fluorescence PCR.The qRT-PCR results showed that the expression trend was consistent with the results of transcriptome data.In this study we found that the genes related to the metabolism of F.solani expressed differentially with significance during the pathogen infecting sweetpotato.The possible reason is that the pathogen needs a lot of nutrients,and then convert them to energy and intermediate,to break out the host defense and colonize in sweetpotato at last.

关 键 词：腐皮镰刀菌 甘薯 转录组 

分 类 号：S531[农业科学—作物学]