陕西省西洋参根结线虫病的发生和病原鉴定  

Pathogen Identification of Panax quinquefolium Root-knot Nematode Disease in Shaanxi of China

作　　者：耿英杰 陈德经[2] 商文静 GENG Yingjie;CHEN Dejing;SHANG Wenjing(College of Plant Protection,Northwest A&F University,Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau,Ministry of Agriculture,Yangling Shaanxi 712100,China;State Key Laboratory of Qinba Biological Resources,Ecological Environment,School of Bioscience and Engineering,Shaanxi University of Technology,Hanzhong Shaanxi 723001,China)

机构地区：[1]西北农林科技大学植物保护学院,农业部西北黄土高原作物有害生物综合治理重点实验室,陕西杨凌712100 [2]秦巴生物资源与生态环境省部共建国家重点实验室,陕西理工大学生物科学与工程学院,陕西汉中723001

出　　处：《西北农业学报》2023年第5期799-806,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省区域创新能力引导计划(2020QFY12-06);秦巴生物资源与生态环境省部共建国家重点实验室开放基金(SLGPT2019KF04-03);西北农林科技大学试验示范站(基地)科技成果推广项目(TGZX2019-32)。

摘　　要：为明确陕西省西洋参根结线虫病病原,采用常规形态鉴定结合分子生物学方法,对在陕西省留坝县严重发生的西洋参根结线虫病进行病原鉴定。结果表明:二龄幼虫蠕虫形,口针基部球小而圆,尾部透明区明显,尾尖钝圆;雌虫虫体梨形或近椭圆形,口针杆部后部稍宽,口针基部球近圆形较小有缢缩。雌虫会阴花纹呈近圆形,线纹平滑呈波浪状,尾端区有刻点,背弓较为低平。结合特异性PCR扩增及根结线虫属rDNA-ITS序列系统发育树构建结果,该病原鉴定为北方根结线虫Meloidogyne hapla。In order to clarify the pathogen species of Panax quinquefolium root-knot nematode disease in Shaanxi,the root samples with obvious root-knots of Panax quinquefolium were collected for isolating root-knot nematodes from P.quinquefolium planting area in Liuba County,Shaanxi Province.The pathogenic nematodes were identified by observing morphological characteristics of females,second-stage juveniles and perineal pattern,sequence alignments,phylogenetic analysis and specific PCR amplification.The results showed that the pathogen species of P.quinquefolium root-knot nematode disease in Shaanxi was M.hapla.

关 键 词：西洋参 北方根结线虫 病原鉴定 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]