A gap-free and haplotype-resolved lemon genome provides insights into flavor synthesis and huanglongbing (HLB) tolerance  

作　　者：Yixue Bao Ziyan Zeng Wei Yao Xiao Chen Mengwei Jiang Akbar Sehrish Bo Wu Charles A.Powell Baoshan Chen Jianlong Xu Xingtan Zhang Muqing Zhang 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agric-Biological Resources,Guangxi University,Nanning 530005,China [2]Hainan Yazhou Bay Seed Laboratory,National Nanfan Research Institute(Sanya),Chinese Academy of Agricultural Sciences,Sanya 572024,China/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China [4]School of Computing,Clemson University,821 McMillan Rd,Clemson,SC 29631,USA [5]IRREC-IFAS,University of Florida,Fort Pierce,FL 34945,USA

出　　处：《Horticulture Research》2023年第4期13-22,共10页园艺研究（英文）

基　　金：supported by the Guangxi Major Project of Science and Technology(Guike AA18118027);the Postdoctoral Project of Hainan Yazhou Bay Seed Laboratory Program(B21Y10203);the Scientific Research and Development Fund of the College of Agriculture,Guangxi University(EE101731).

摘　　要：The lemon(Citrus limon;family Rutaceae)is one of the most important and popular fruits worldwide.Lemon also tolerates huan-glongbing(HLB)disease,which is a devastating citrus disease.Here we produced a gap-free and haplotype-resolved chromosome-scale genome assembly of the lemon by combining Pacific Biosciences circular consensus sequencing,Oxford Nanopore 50-kb ultra-long,and high-throughput chromatin conformation capture technologies.The assembly contained nine-pair chromosomes with a contig N50 of 35.6 Mb and zero gaps,while a total of 633.0 Mb genomic sequences were generated.The origination analysis identified 338.5Mb genomic sequences originating from citron(53.5%),147.4Mb frommandarin(23.3%),and 147.1Mb frompummelo(23.2%).The genome included 30528 protein-coding genes,and most of the assembled sequences were found to be repetitive sequences.Several significantly expanded gene families were associated with plant-pathogen interactions,plant hormone signal transduction,and the biosynthesis of major active components,such as terpenoids and f lavor compounds.Most HLB-tolerant genes were expanded in the lemon genome,such as 2-oxoglutarate(2OG)/Fe(II)-dependent oxygenase and constitutive disease resistance 1,cell wall-related genes,and lignin synthesis genes.Comparative transcriptomic analysis showed that phloem regeneration and lower levels of phloem plugging are the elements that contribute to HLB tolerance in lemon.Our results provide insight into lemon genome evolution,active component biosynthesis,and genes associated with HLB tolerance.

关 键 词：SYNTHESIS insight RESOLVED 

分 类 号：S666.5[农业科学—果树学]