Generating colorful carrot germplasm through metabolic engineering of betalains pigments  

作　　者：Yuan-Jie Deng Ao-Qi Duan Hui Liu Ya-Hui Wang Rong-Rong Zhang Zhi-Sheng Xu Ai-Sheng Xiong 

机构地区：[1]National Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization,Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,College of Horticulture,Nanjing Agricultural University,1 Weigang,Nanjing 210095,China

出　　处：《Horticulture Research》2023年第4期63-70,共8页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(32072563);Key Research and Development Projects of Ningxia Hui Autonomous Region(No.2022BBF02008);Priority Academic Program Development of Jiangsu Higher Education Institutions(Grant No.PAPD)。

摘　　要：Betalains are tyrosine-derived plant pigments exclusively found in the Caryophyllales order and some higher fungi and generally classified into two groups:red-violet betacyanins and yellow-orange betaxanthins.Betalains attract great scientific and economic interest because of their relatively simple biosynthesis pathway,attractive colors and health-promoting properties.Co-expressing two core genes BvCYP76AD1 and BvDODA1 with or without a glycosyltransferase gene MjcDOPA5GT allowed the engineering of carrot(an important taproot vegetable)to produce a palette of unique colors.The highest total betalains content,943.2μg·g^(−1) DW,was obtained in carrot taproot transformed with p35S:RUBY which produces all of the necessary enzymes for betalains synthesis.Root-specific production of betalains slightly relieved tyrosine consumption revealing the possible bottleneck in betalains production.Furthermore,a unique volcano-like phenotype in carrot taproot cross-section was created by vascular cambium-specific production of betalains.The betalains-fortified carrot in this study is thus anticipated to be used as functional vegetable and colorful carrot germplasm in breeding to promote health.

关 键 词：GERMPLASM breeding ORANGE 

分 类 号：S631.2[农业科学—蔬菜学]