Novel flavin-containing monooxygenase protein FMO1 interacts with CAT2 to negatively regulate drought tolerance through ROS homeostasis and ABA signaling pathway in tomato  

作　　者：Lulu Wang Yinlian Zhou Yin Ding Chunrui Chen Xueting Chen Nini Su Xingguo Zhang Yu Pan Jinhua Li 

机构地区：[1]State Cultivation Base of Crop Stress Biology for Southern Mountainous land of Southwest University,Academy of Agricultural Sciences,Southwest University,Beibei,Chongqing 400715,China [2]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education),College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China

出　　处：《Horticulture Research》2023年第4期225-237,共13页园艺研究（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(No.31872123);Natural Science Foundation of Chongqing,China(No.cstc2019jcyj-msxmX0333);the Fundamental Research Funds for the Central Universities(No.XDJK2020B060).

摘　　要：Drought stress is themajor abiotic factor that can seriously affect plant growth and crop production.The functions of flavin-containing monooxygenases(FMOs)are known in animals.They addmolecular oxygen to lipophilic compounds or produce reactive oxygen species(ROS).However,little information on FMOs in plants is available.Here,we characterized a tomato drought-responsive gene that showed homology to FMO,and it was designated as FMO1.FMO1 was downregulated promptly by drought and ABA treatments.Transgenic functional analysis indicated that RNAi suppression of the expression of FMO1(FMO1-Ri)improved drought tolerance relative to wild-type(WT)plants,whereas overexpression of FMO1(FMO1-OE)reduced drought tolerance.The FMO1-Ri plants exhibited lower ABA accumulation,higher levels of antioxidant enzyme activities,and less ROS generation comparedwith theWTand FMO1-OE plants under drought stress.RNA-seq transcriptional analysis revealed the differential expression levels of many drought-responsive genes thatwere co-expressed with FMO1,including PP2Cs,PYLs,WRKY,and LEA.Using Y2H screening,we found that FMO1 physically interacted with catalase 2(CAT2),which is an antioxidant enzyme and confers drought resistance.Our findings suggest that tomato FMO1 negatively regulates tomato drought tolerance in the ABA-dependent pathway and modulates ROS homeostasis by directly binding to SlCAT2.

关 键 词：mono drought TRANSGENIC 

分 类 号：Q94[生物学—植物学]