Citrus β-carotene hydroxylase 2 (BCH2) participates in xanthophyll synthesis by catalyzing the hydroxylation of β-carotene and compensates for BCH1 in citrus carotenoid metabolism  

作　　者：Yingzi Zhang Jiajing Jin Shenchao Zhu Quan Sun Yin Zhang Zongzhou Xie Junli Ye Xiuxin Deng 

机构地区：[1]Key Laboratory of Horticultural Plant Biology(Ministry of Education),Huazhong Agricultural University,Wuhan,430070,China

出　　处：《Horticulture Research》2023年第3期45-56,共12页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(No.31930095 and 32172527);the Modern Agro-industry Technology Research System(CARS-26).

摘　　要：Abstract As an essential horticultural crop,Citrus has carotenoid diversity,which affects its aesthetic and nutritional values.β,β-Xanthophylls are the primary carotenoids accumulated in citrus fruits,and non-heme di-iron carotene hydroxylase(BCH)enzymes are mainly responsible forβ,β-xanthophyll synthesis.Previous studies have focused on the hydroxylation of BCH1,but the role of its paralogous gene in citrus,BCH2,remains largely unknown.In this study,we revealed theβ-hydroxylation activity of citrus BCH2(CsBCH2)for the first time through the functional complementation assay using Escherichia coli,although CsBCH2 exhibited a lower activity in hydroxylatingβ-carotene intoβ-cryptoxanthin than citrus BCH1(CsBCH1).Our results showed that overexpression of CsBCH2 in citrus callus increased xanthophyll proportion and plastoglobule size with feedback regulation of carotenogenic gene expression.This study revealed the distinct expression patterns and functional characteristics of two paralogous genes,CsBCH1 and CsBCH2,and illustrated the backup compensatory role of CsBCH2 for CsBCH1 in citrus xanthophyll biosynthesis.The independent function of CsBCH2 and its cooperative function with CsBCH1 inβ-cryptoxanthin biosynthesis suggested the potential of CsBCH2 to be employed for expanding the synthetic biology toolkit in carotenoid engineering。

关 键 词：CITRUS CITRUS METABOLISM 

分 类 号：S66[农业科学—果树学]