Jasmonic acid regulates the biosynthesis of medicinal metabolites via the JAZ9-MYB76 complex in Salvia miltiorrhiza  

作　　者：Shucan Liu Xiankui Gao Min Shi Meihong Sun Kunlun Li Yan Cai Chengan Chen Can Wang Itay Maoz Xinhong Guo Guoyin Kai 

机构地区：[1]Laboratory of Medicinal Plant Biotechnology,School of Pharmaceutical Sciences,Jinhua Academy,Zhejiang Chinese Medical University,Hangzhou,Zhejiang 310053,China [2]College of Biology,Hunan University,Changsha,Hunan 410082,China [3]Institute of Plant Biotechnology,School of Life Sciences,Shanghai Normal University,Shanghai 200234,China [4]Department of Postharvest Science,ARO,The Volcani Center,HaMaccabim Rd 68,POB 15159,Rishon LeZion,7528809,Israel

出　　处：《Horticulture Research》2023年第3期110-123,共14页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(82073963,81522049);The Key Science and Technology Projects of Breeding New Varieties of Agriculture in Zhejiang Province(2021C02074-3);Zhejiang Provincial Ten Thousands Program for Leading Talents of Science and Technology Innovation(2018R52050);Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents;the Research Project of Zhejiang Chinese Medical University(2021JKZDZC06).

摘　　要：Jasmonic acid(JA)signaling pathway plays an important role in tanshinone and phenolic acid biosynthesis in Salvia miltiorrhiza.However,the specific regulatory mechanism remains largely unclear.Previous work showed that a JASMONATE ZIM-domain(JAZ)protein,SmJAZ9,acted as a repressor of tanshinone production in S.miltiorrhiza.In this study,we revealed that SmJAZ9 reduced both phenolic acid accumulation and related biosynthetic gene expression,confirming that SmJAZ9 also negatively affected phenolic acid biosynthesis.Then,we identified a novel MYB transcription factor,SmMYB76,which interacted with SmJAZ9.SmMYB76 repressed phenolic acid biosynthesis by directly downregulating SmPAL1,Sm4CL2,and SmRAS1.Further investigation demonstrated that JA mediated phenolic acids biosynthesis via SmJAZ9-SmMYB76 complex.Taken together,these findings state the molecular mechanism that SmJAZ9-SmMYB76 regulated phenolic acid biosynthesis at the transcriptional and protein levels,which provided new insights into JA signaling pathway regulating plant metabolism.

关 键 词：COMPLEX PHENOLIC INSIGHT 

分 类 号：S685.16[农业科学—观赏园艺]