Genome-wide association study of 23 f lowering phenology traits and 4 f loral agronomic traits in tree peony (Paeonia section Moutan DC.) reveals five genes known to regulate f lowering time  

作　　者：Yuying Li Lili Guo Zhanying Wang Dehui Zhao Dalong Guo John E.Carlson Weilun Yin Xiaogai Hou 

机构地区：[1]College of Agronomy/College of Tree Peony,Henan University of Science and Technology,Luoyang,Henan,471023,China [2]Luoyang Academy of Agricultural and Forestry Sciences,Luoyang,Henan,471000,China [3]College of Forestry,Henan University of Science and Technology,Luoyang,Henan,471023,China [4]Department of Ecosystem Science and Management,Pennsylvania State University,University Park,PA 16802,USA [5]College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China

出　　处：《Horticulture Research》2023年第2期118-133,共16页园艺研究（英文）

基　　金：supported by the National Natural Science Foundation of China(U1804233);the Innovation Scientists and Technicians Troop Construction Projects of Henan Province(202101510003);the Outstanding Youth Fund of the Natural Science Foundation of Henan Province(202300410119).

摘　　要：Tree peony is a unique traditional f lower in China,with large,fragrant,and colorful f lowers.However,a relatively short and concentrated f lowering period limits the applications and production of tree peony.A genome-wide association study(GWAS)was conducted to accelerate molecular breeding for the improvement of f lowering phenology traits and ornamental phenotypes in tree peony.A diverse panel of 451 tree peony accessions was phenotyped for 23 f lowering phenology traits and 4 f loral agronomic traits over 3 years.Genotyping by sequencing(GBS)was used to obtain a large number of genome-wide single-nucleotide polymorphisms(SNPs)(107050)for the panel genotypes,and 1047 candidate genes were identified by association mapping.Eighty-two related genes were observed during at least 2 years for f lowering,and seven SNPs repeatedly identified for multiple f lowering phenology traits over multiple years were highly significantly associated with five genes known to regulate f lowering time.We validated the temporal expression profiles of these candidate genes and highlighted their possible roles in the regulation of f lower bud differentiation and f lowering time in tree peony.This study shows that GWAS based on GBS can be used to identify the genetic determinants of complex traits in tree peony.The results expand our understanding of f lowering time control in perennial woody plants.Identification of markers closely related to these f lowering phenology traits can be used in tree peony breeding programs for important agronomic traits.

关 键 词：breeding TRAITS tree 

分 类 号：S685.11[农业科学—观赏园艺]