Genome assembly of wild loquat (Eriobotrya japonica) and resequencing provide new insights into the genomic evolution and fruit domestication in loquat  

作　　者：Danlong Jing Xinya Liu Qiao He Jiangbo Dang Ruoqian Hu Yan Xia Di Wu Shuming Wang Yin Zhang Qingqing Xia Chi Zhang Yuanhui Yu Qigao Guo Guolu Liang 

机构地区：[1]Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China [2]Academy of Agricultural Sciences of Southwest University,State Cultivation Base of Crop Stress Biology for Southern Mountainous Land,Chongqing 400715,China

出　　处：《Horticulture Research》2023年第2期147-159,共13页园艺研究（英文）

基　　金：supported by the National Key R&D Program of China(No.2019YFD1000200);the National Nature Science Foundation of China(No.32102321);the Chongqing Science and Technology Commission(cstc2021jcyj-msxmX1156 and cstc2021jscxgksbX0010);the Innovation Research Group Funds for Chongqing Universities(CXQT19005);the Fundamental Research Funds for the Central Universities(SWU-KT22055).

摘　　要：Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding genes.Analysis of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its divergence.Genome resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic admixture.Compared with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic diversity.However,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication process.Large-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development stages.Unlike those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit development.These high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.

关 键 词：process insight breeding 

分 类 号：S667.3[农业科学—果树学]