寄主营养和次生代谢物质对牛角花齿蓟马(缨翅目:蓟马科)产卵选择的影响  

Effects of host plant nutrients and secondary metabolites on the oviposition selectivity of Odontothrips loti(Thysanoptera:Thripidae)

作　　者：崔晓宁 席驳鑫 赵晓东 胡桂馨[1] 李昌宁 张博鸿 彭斌 CUI Xiaoning;XI Boxin;ZHAO Xiaodong;HU Guixin;LI Changning;ZHANG Bohong;PENG Bin(Pratacultural College,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Engineering and Technology Research Center for Alpine Rodent Pests Control,National Forestry and Grassland Administration,Lanzhou 730070,China;College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China;Kongtong County Agriculture and Rural Bureau,Pingliang City,Gansu Province,Pingliang 744000,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,国家林业草原高寒草地鼠害防控工程技术研究中心,兰州730070 [2]甘肃农业大学植物保护学院,兰州730070 [3]甘肃省平凉市崆峒区农业农村局,平凉744000

出　　处：《植物保护》2023年第3期92-99,共8页Plant Protection

基　　金：国家自然科学基金(32060397);甘肃农业大学博士科研启动基金(GAU-KYQD-2018-24);国家牧草产业技术体系(CARS-34)。

摘　　要：为探究牛角花齿蓟马Odontothrips loti的产卵选择及寄主营养和次生代谢物质对其产卵行为的影响,本研究观测了牛角花齿蓟马在苜蓿Medicago sativa、草木樨Melilotus officinalis、红豆草Onobrychis cyri和红三叶Trifolium pratense 4种豆科牧草叶片上的产卵量和卵孵化率,同时测定、分析了牧草主要营养和次生代谢物质含量与蓟马产卵选择的相关性。结果表明,无选择条件下,牛角花齿蓟马在苜蓿上产卵量最高,为59.1粒/复叶,其次是草木樨,15.7粒/复叶,在红豆草和红三叶上的产卵量仅为0.9粒/复叶和1.1粒/复叶。卵孵化率在苜蓿上最高,为85.05%,在草木樨和红豆草上分别为40.09%和39.18%,红三叶上最低,为5.48%。两两选择条件下,牛角花齿蓟马明显偏好在苜蓿叶片上产卵,在其他牧草叶片上不产卵或产卵量极低。相关性分析表明,牛角花齿蓟马的产卵量与寄主可溶性蛋白含量显著正相关(r=0.722,P=0.002),卵孵化率与可溶性蛋白(r=0.673,P=0.004)和淀粉(r=0.586,P=0.017)含量显著正相关。牛角花齿蓟马产卵量分别与寄主总酚(r=-0.771,P<0.001)、简单酚(r=-0.724,P=0.002)、单宁(r=-0.755,P=0.001)和黄酮(r=-0.672,P=0.004)4种次生代谢物质含量呈显著负相关性。说明寄主叶片的可溶性蛋白含量高有利于牛角花齿蓟马产卵,总酚、简单酚、单宁和黄酮含量高,对蓟马产卵具有抑制作用。This study aimed to clarify the oviposition preference of Odontothrips loti to different legumes,and the effects of host nutrients and secondary metabolites on its oviposition behavior.The oviposition capacity and egg hatchability of thrips on leaves of four different legume forages,including Medicago sativa,Melilotus officinalis,Onobrychis cyri and Trifolium pratense,were measured.Meanwhile,the contents of main nutrients and secondary metabolites of forages were determined,and their correlations with oviposition selectivity of thrips were also analyzed.The no-choice test results showed that the largest amount of oviposition was 59.1 eggs per compound leaf on M.sativa,followed by on M.officinalis with 15.7 eggs per leaf,and the smallest on O.cyri and T.pratense,with 0.9 and 1.1 eggs per leaf,respectively.The highest egg hatching rate was 85.05%on M.sativa,followed by 40.09%on M.officinalis,39.18%on O.cyri,and 5.48%on T.pratense.In dual-choice tests,Odontothrips loti obviously preferred to lay eggs on M.sativa leaves,but there were few eggs laid on the other host plants.Correlation analysis showed that the egg-laying amounts were significantly positively correlated with the content of leaf soluble proteins(r=0.722,P=0.002).The egg-hatching rate was significantly positively correlated with the content of leaf soluble proteins(r=0.673,P=0.004)and starch(r=0.586,P=0.017),respectively.However,the egg-laying amounts were significantly negatively correlated with the content of total phenol(r=-0.771,P<0.001),simple phenol(r=-0.724,P=0.002),tannin(r=-0.755,P=0.001)and flavonoid(r=-0.672,P=0.004)in legume leaves,respectively.This study suggested that O.loti preferred to lay eggs on host plants with higher content of soluble proteins and less contents of total phenol,simple phenol,tannin and flavonoid.

关 键 词：牛角花齿蓟马 苜蓿 寄主植物 产卵选择 营养物质 次生代谢物质 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]