孤雌产雌食胚赤眼蜂高温敏感阶段的研究  

Sensitive stages of thelytokous parthenogenetic Trichogramma embryophagum infected by Wolbachia in response to high temperature

作　　者：李明睿 王震刚 刘婷 匡悦 王强[1] 李迎瑞 李博洋 刘向涛 吴雨聪 张海燕[1,2] LI Mingrui;WANG Zhengang;LIU Ting;KUANG Yue;WANG Qiang;LI Yingrui;LI Boyang;LIU Xiangtao;WU Yucong;ZHANG Haiyan(College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Heilongjiang Provincial Research Center for Engineering Technology of Straw Resource Utilization,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院,大庆163319 [2]黑龙江省秸秆资源化利用工程技术研究中心,大庆163319

出　　处：《植物保护》2023年第3期100-104,112,共6页Plant Protection

基　　金：国家级大学生创新创业训练计划(202010223046);国家自然科学基金青年科学基金(31301714);黑龙江省自然科学基金(C2015039);校级留学归国计划项目(ZRCLG201901)。

摘　　要：胞内共生菌Wolbachia可诱导赤眼蜂产雌孤雌生殖,而高温可使感染Wolbachia的赤眼蜂产雄比例增加。本文以感染Wolbachia孤雌产雌生殖的食胚赤眼蜂Trichogramma embryophagum为材料,采用30℃和35℃高温处理卵、幼虫、预蛹、蛹和成虫等不同发育阶段的虫体,统计子代蜂发育历期、寄生数、羽化率和性比。结果表明,与25℃对照相比,高温缩短了子代蜂各发育阶段的历期,其中蛹、成虫和全世代历期显著缩短。处理的发育阶段和持续时间不同,子代的出雄率不同,而且随着处理时间的延长,对食胚赤眼蜂子代的寄生和羽化有一定的影响。基于高温处理后子代出雄率指标分析,F3代卵和幼虫经高温处理后代子代雄蜂比率最高,达到了20.54%和23.78%,明显高于预蛹期和蛹期处理的雄蜂率。结果表明,高温处理后感染Wolbachia的食胚赤眼蜂生殖方式改变的敏感发育阶段为卵期和幼虫期,本研究可为进一步揭示Wolbachia调控赤眼蜂生殖的机理提供理论参考。The intracellular symbiotic Wolbachia can induce thelytokous parthenogenesis in Trichogramma,but the proportion of male offsprings of Trichogramma infected with Wolbachia will increase after treatment with higher temperature.In this study,the eggs,larvae,prepupae,pupae and adults of infected Trichogramma embryophagum were treated at 30℃and 35℃.The developmental duration,parasitism number,emergence rate and sex ratio of progenies were calculated.The results showed that high temperature shortened the developmental duration of the offspring,especially the developmental durations of pupa,adult and whole generation.The percentages of offspring males were different with different developmental stages treated and different treatment durations.The parasitism and emergence were changed with increasing treatment time.Based on the analysis of the percentages of offspring males under high-temperature treatment,the highest percentages of males were recorded when eggs and larvae were treated in F 3 generation,reaching 20.54%and 23.78%,respectively,higher than those when pre-pupae and pupae were treated.The results suggested that the changes of reproductive pattern of T.embryophagum induced by Wolbachia were most sensitive to high-temperature treatment at egg and larval stages.This study provides a theoretical reference for further revealing the reproductive mechanism of Trichogramma associated with Wolbachia infection.

关 键 词：WOLBACHIA 食胚赤眼蜂 孤雌产雌生殖 发育阶段 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]