牡丹皮提取物除草活性初步研究  

A preliminary study of the herbicidal activity of the extracts from Cortex Moutan Radicis

作　　者：贾然 田赐 李晶晶 封英建 赵雪萍 牛文韬 马树杰 张利辉[1] 董金皋[1] JIA Ran;TIAN Ci;LI Jingjing;FENG Yingjian;ZHAO Xueping;NIU Wentao;MA Shujie;ZHANG Lihui;DONG Jingao(College of Plant Protection,Hebei Agricultural University,Baoding 071001,China;Xingtai Modern Vocational School,Xingtai 054000,China)

机构地区：[1]河北农业大学植物保护学院,保定071001 [2]邢台现代职业学校,邢台054000

出　　处：《植物保护》2023年第3期135-140,共6页Plant Protection

基　　金：河北省自然科学基金(C2020204036)。

摘　　要：为明确牡丹皮(Cortex Moutan Radicis)的除草活性并初步探索其活性成分分离条件,采用平皿法、土壤喷雾法及茎叶喷雾法测定了牡丹皮不同极性溶剂萃取物对反枝苋Amaranthus retroflexus和稗Echinochloa crus-galli的除草活性。平皿法测定结果显示,在1 mg/mL浓度下,牡丹皮正丁醇萃取物的除草活性最强,对反枝苋幼根和幼茎的抑制率分别为67.88%和65.71%,对稗草幼根和幼茎的抑制率分别为68.72%和71.32%;毒力测定结果表明,牡丹皮正丁醇萃取物对反枝苋幼根和幼茎的IC50分别为12.28 mg/L和19.67 mg/L,对稗草幼根和幼茎的IC50分别为43.35 mg/L和72.79 mg/L。土壤喷雾法和茎叶喷雾法测定结果显示,在5 mg/mL浓度下,牡丹皮正丁醇萃取物对反枝苋和稗草的鲜重抑制率最高,其中土壤喷雾法对反枝苋和稗草的鲜重抑制率分别为81.43%和83.18%;茎叶喷雾法对两种杂草的鲜重抑制率分别为80.83%和83.65%。由此可见,牡丹皮的除草活性成分主要集中在正丁醇萃取物中,值得进一步研究。In order to clarify the herbicidal activity of Cortex Moutan Radicis and preliminarily explore the separation conditions,the herbicidal activities of different extracts from the Cortex Moutan Radicis against Amaranthus retroflexus and Echinochloa crus-galli were determined by bioactivity assay in the laboratory.The plating method showed that the n-butanol extracts of the Cortex Moutan Radicis(1 mg/mL)had the strongest herbicidal activity,with an inhibition rate of 67.88%and 65.71%against young roots and stems of A.retroflexus,and 68.72%and 71.32%against young roots and stems of E.crus-galli,respectively.Further toxicity assays showed that the IC 50 values of n-butanol extracts of Cortex Moutan Radicis were 12.28 mg/L and 19.67 mg/L against young roots and stems of A.retroflexus,43.35 mg/L and 72.79 mg/L against E.crus-galli,respectively.The soil spray method showed that the n-butanol extracts of Cortex Moutan Radicis(5 mg/mL)had the strongest herbicidal activity,with a fresh weight inhibition rate of 81.43%and 83.18%against A.retroflexus and E.crus-galli,respectively.The n-butanol extracts of Cortex Moutan Radicis also had the strongest activity by using the stem-leaf spray method,with a fresh weight inhibition rate of 80.83%and 83.65%against A.retroflexus and E.crus-galli,respectively.It suggested that the herbicidal active components of Cortex Moutan Radicis are mainly concentrated in the n-butanol extracts,and the active compounds are worth further investigation.

关 键 词：牡丹皮萃取物 反枝苋 稗 除草活性 植物源农药 

分 类 号：S482.49[农业科学—农药学]