辽宁梨树枝枯病病原菌Diplodia seriata的分离与鉴定  

Isolation and identification of the pathogen Diplodia seriata causing stem blight disease on pear in Liaoning

作　　者：丁丹丹 刘博 李爽 赵继荣 程国亭 回虹燕 徐成楠 DING Dandan;LIU Bo;LI Shuang;ZHAO Jirong;CHENG Guoting;HUI Hongyan;XU Chengnan(College of Life Science,Yan’an University,Shaanxi Key Laboratory of Chinese Jujube,Yan’an 716000,China)

机构地区：[1]延安大学生命科学学院,陕西省红枣重点实验室,延安716000

出　　处：《植物保护》2023年第3期226-230,共5页Plant Protection

基　　金：陕西省重点研发计划(2021NY-065);延安大学博士科研启动项目(YDBK2019-45);延安大学2020年科研计划(YDQ2020-16);延安市2021年重点研发计划(2021XY-12);延安大学2019年校级大学生创新创业训练计划。

摘　　要：在辽宁兴城秋子梨栽培园出现一种新型枝干病害,主要症状为枝枯及木质部坏死。为明确梨树枝枯病病原菌的分类地位,本研究从辽宁兴城地区采样,经过菌株分离培养和致病性测定,以及形态学鉴定结合病原菌的ITS、EF-1α和β-tubulin多基因联合构建系统发育树,确定引起秋子梨枝枯病的病原菌为色二孢Diplodia seriata。研究结果为明确色二孢引起的辽宁梨树枝枯病发生规律和防治措施的制定提供了理论基础。A new stem disease on Pyrus ussuriensis orchard with typical symptoms including branches dieback and xylem necrosis was observed in Xingcheng city,Liaoning province.In order to clarify the pathogen causing stem blight disease on pear in Liaoning,samples were collected,meanwhile,isolates were purified and Koch’s postulation was carried out.Based on morphological features and combined gene sequence analysis on ITS,EF-1αandβ-tubulin,the causal pathogen of this new disease was identified to be Diplodia seriata.Our results provided a theoretical basis for further research on the disease epidemic and control strategy of stem blight disease on P.ussuriensis in Liaoning.

关 键 词：梨树 枝枯病 系统发育分析 色二孢 

分 类 号：S436.612[农业科学—农业昆虫与害虫防治]