Diploid chromosome-level reference genome and population genomic analyses provide insights into Gypenoside biosynthesis and demographic evolution of Gynostemma pentaphyllum (Cucurbitaceae)  

作　　者：Xiao Zhang Yuhe Zhao Yixuan Kou Xiaodan Chen Jia Yang Hao Zhang Zhe Zhao Yuemei Zhao Guifang Zhao Zhonghu Li 

机构地区：[1]Key Laboratory of Resource Biology and Biotechnology in Western China(Ministry of Education),College of Life Sciences,Northwest University,Xi’an,Shaanxi,710069,China [2]Laboratory of Subtropical Biodiversity,Jiangxi Agricultural University,Nanchang,330045,China [3]College of Life Sciences,Shanxi Normal University,Taiyuan,Shanxi,030012,China [4]College of Life Sciences,Sun Yat-sen University,Guangzhou,Guangdong,510275,China [5]School of Biological Sciences,Guizhou Education University,Guiyang,Guizhou,550018,China

出　　处：《Horticulture Research》2023年第1期82-95,共14页园艺研究（英文）

基　　金：This study was supported by the National Natural Science Foundation of China(Nos.32000256,31900273,31770229,and 31270364);a Project funded by the China Postdoctoral Science Foundation(No.2020M673627XB);the Key Pro-gram of Research and Development of Shaanxi Province(No.2022ZDLSF06-02).

摘　　要：Gynostemma pentaphyllum(Thunb.)Makino is a perennial creeping herbaceous plant in the family Cucurbitaceae,which has great medicinal value and commercial potential,but urgent conservation efforts are needed due to the gradual decreases and fragmented distribution of its wild populations.Here,we report the high-quality diploid chromosome-level genome of G.pentaphyllum obtained using a combination of next-generation sequencing short reads,Nanopore long reads,and Hi-C sequencing technologies.The genome is anchored to 11 pseudo-chromosomes with a total size of 608.95 Mb and 26588 predicted genes.Comparative genomic analyses indicate that G.pentaphyllum is estimated to have diverged from Momordica charantia 60.7 million years ago,with no recent whole-genome duplication event.Genomic population analyses based on genotyping-by-sequencing and ecological niche analyses indicated low genetic diversity but a strong population structure within the species,which could classify 32 G.pentaphyllum populations into three geographical groups shaped jointly by geographic and climate factors.Furthermore,comparative transcriptome analyses showed that the genes encoding enzyme involved in gypenoside biosynthesis had higher expression levels in the leaves and tendrils.Overall,the findings obtained in this study provide an effective molecular basis for further studies of demographic genetics,ecological adaption,and systematic evolution in Cucurbitaceae species,as well as contributing to molecular breeding,and the biosynthesis and biotransformation of gypenoside.

关 键 词：EVOLUTION jointly breeding 

分 类 号：Q93[生物学—微生物学]