Resequencing of sweetpotato germplasm resources reveals key loci associated with multiple agronomic traits  

作　　者：Shizhuo Xiao Xibin Dai Lingxiao Zhao Zhilin Zhou Lukuan Zhao Pan Xu Bingqian Gao An Zhang Donglan Zhao Rui Yuan Yao Wang Jie Wang Qinglian Li Qinghe Cao 

机构地区：[1]Jiangsu Xuzhou Sweetpotato Research Center/Sweetpotato Research Institute,Chinese Agricultural Academy of Sciences,Xuzhou 221131,China [2]College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China

出　　处：《Horticulture Research》2023年第1期128-139,共12页园艺研究（英文）

基　　金：This work was supported by the National Key Research&Development Program of China(2018YFD1000705/2018YFD1000700);the Natural Science Foundation of Jiangsu Province of China(BK20221213);the China Agriculture Research System(CARS-10-GW01).

摘　　要：Sweetpotato is an important crop that exhibits hexaploidy and high heterozygosity,which limits gene mining for important agronomic traits.Here,314 sweetpotato germplasm resources were deeply resequenced,and 4599509 SNPs and 846654 InDels were generated,among which 196124 SNPs were nonsynonymous and 9690 InDels were frameshifted.Based on the Indels,genome-wide marker primers were designed,and 3219 of 40366 primer pairs were selected to construct the core InDel marker set.The molecular ID of 104 sweetpotato samples verified the availability of these primers.The sweetpotato population structures were then assessed through multiple approaches using SNPs,and diverse approaches demonstrated that population stratification was not obvious for most Chinese germplasm resources.As many as 20 important agronomic traits were evaluated,and a genome-wide association study was conducted on these traits.A total of 19 high-confidence loci were detected in both models.These loci included several candidate genes,such as IbMYB1,IbZEP1,and IbYABBY1,which might be involved in anthocyanin metabolism,carotenoid metabolism,and leaf morphogenesis,respectively.Among them,IbZEP1 and IbYABBY1 were first reported in sweetpotato.The variants in the promoter and the expression levels of IbZEP1 were significantly correlated with f lesh color(orange or not orange)in sweetpotato.The expression levels of IbYABBY1 were also correlated with leaf shape.These results will assist in genetic and breeding studies in sweetpotato.

关 键 词：GERMPLASM breeding traits 

分 类 号：S531[农业科学—作物学]