Gapless genome assembly of azalea and multi-omics investigation into divergence between two species with distinct f lower color  

作　　者：Shuai Nie Shi-Wei Zhao Tian-Le Shi Wei Zhao Ren-Gang Zhang Xue-Chan Tian Jing-Fang Guo Xue-Mei Yan Yu-Tao Bao Zhi-Chao Li Lei Kong Hai-Yao Ma Zhao-Yang Chen Hui Liu Yousry AEl-Kassaby Ilga Porth Fu-Sheng Yang Jian-Feng Mao 

机构地区：[1]National Engineering Research Center of Tree Breeding and Ecological Restoration,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083,China [2]Department of Ecology and Environmental Science,UmeåPlant Science Centre,UmeåUniversity,SE-90187 Umeå,Sweden [3]Department of Bioinformatics,Ori(Shandong)Gene Science and Technology Co.,Ltd.,Weifang 261322,China [4]Department of Forest and Conservation Sciences,Faculty of Forestry,University of British Columbia,Vancouver,BC,V6T 1Z4,Canada [5]Départment des Sciences du Bois et de la Forêt,Facultéde Foresterie,de Géographie et Géomatique,UniversitéLaval,Québec,QC,G1V 0A6,Canada [6]State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [7]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Horticulture Research》2023年第1期193-206,共14页园艺研究（英文）

基　　金：This work was supported by grants from the Strategic Prior-ity Research Program,Chinese Academy of Sciences(Grant No.XDA23080000);Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502).

摘　　要：The genus Rhododendron(Ericaceae),with more than 1000 species highly diverse in f lower color,is providing distinct ornamental values and a model system for f lower color studies.Here,we investigated the divergence between two parental species with different f lower color widely used for azalea breeding.Gapless genome assembly was generated for the yellow-f lowered azalea,Rhododendron molle.Comparative genomics found recent proliferation of long terminal repeat retrotransposons(LTR-RTs),especially Gypsy,has resulted in a 125 Mb(19%)genome size increase in species-specific regions,and a significant amount of dispersed gene duplicates(13402)and pseudogenes(17437).Metabolomic assessment revealed that yellow f lower coloration is attributed to the dynamic changes of carotenoids/f lavonols biosynthesis and chlorophyll degradation.Time-ordered gene co-expression networks(TO-GCNs)and the comparison confirmed the metabolome and uncovered the specific gene regulatory changes underpinning the distinct f lower pigmentation.B3 and ERF TFs were found dominating the gene regulation of carotenoids/f lavonols characterized pigmentation in R.molle,while WRKY,ERF,WD40,C2H2,and NAC TFs collectively regulated the anthocyanins characterized pigmentation in the red-f lowered R simsii.This study employed a multi-omics strategy in disentangling the complex divergence between two important azaleas and provided references for further functional genetics and molecular breeding.

关 键 词：DIVERGENCE YELLOW COLLECTIVE 

分 类 号：S685.21[农业科学—观赏园艺]