High-throughput discovery of plastid genes causing albino phenotypes in ornamental chimeric plants  

作　　者：Hyun-Seung Park Jae-Hyeon Jeon Woohyeon Cho Yeonjeong Lee Jee Young Park Jiseok Kim Young Sang Park Hyun Jo Koo Jung Hwa Kang Taek Joo Lee Sang Hoon Kim Jin-Baek Kim Hae-Yun Kwon Suk-Hwan Kim Nam-Chon Paek Geupil Jang Jeong-Yong Suh Tae-Jin Yang 

机构地区：[1]Department of Agriculture,Forestry and Bioresources,Research Institute of Agriculture and Life Sciences,and Plant Genomics and Breeding Institute,College of Agriculture and Life Sciences,Seoul National University,Seoul,08826,Republic of Korea [2]Department of Integrative Biological Sciences and Industry,Sejong University,Seoul 05006,Korea [3]Hantaek Botanical Garden,Yongin,Gyeonggi-do,17183,Republic of Korea [4]Radiation Breeding Research Team,Advanced Radiation Technology Institute,Korea Atomic Energy Research Institute,Jeongeup 56212,Korea [5]Special Forest Resources Division,National Institute of Forest Science,Suwon 16631,Korea [6]School of Biological Sciences and Technology,Chonnam National University,Gwangju,South Korea [7]Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences,Seoul National University,Seoul 08826,Korea

出　　处：《Horticulture Research》2023年第1期249-259,共11页园艺研究（英文）

基　　金：This work was supported by the Bio&Medical Technology Development Program of the NRF,MSIP,Republic of Korea(grant no.NRF-2015M3A9A5030733);grants from the Nuclear R&D Program of the Ministry of Science and ICT(MSIT)and the research program of KAERI,Republic of Korea.

摘　　要：Chimeric plants composed of green and albino tissues have great ornamental value.To unveil the functional genes responsible for albino phenotypes in chimeric plants,we inspected the complete plastid genomes(plastomes)in green and albino leaf tissues from 23 ornamental chimeric plants belonging to 20 species,including monocots,dicots,and gymnosperms.In nine chimeric plants,plastomes were identical between green and albino tissues.Meanwhile,another 14 chimeric plants were heteroplasmic,showing a mutation between green and albino tissues.We identified 14 different point mutations in eight functional plastid genes related to plastid-encoded RNA polymerase(rpo)or photosystems which caused albinism in the chimeric plants.Among them,12 were deleterious mutations in the target genes,in which early termination appeared due to small deletion-mediated frameshift or single nucleotide substitution.Another was single nucleotide substitution in an intron of the ycf3 and the other was a missense mutation in coding region of the rpoC2 gene.We inspected chlorophyll structure,protein functional model of the rpoC2,and expression levels of the related genes in green and albino tissues of Reynoutria japonica.A single amino acid change,histidine-to-proline substitution,in the rpoC2 protein may destabilize the peripheral helix of plastid-encoded RNA polymerase,impairing the biosynthesis of the photosynthesis system in the albino tissue of R.japonica chimera plant.

关 键 词：plast substitution ORNAMENTAL 

分 类 号：S601[农业科学—园艺学]