Integration of semi-in vivo assays and multi-omics data reveals the effect of galloylated catechins on self-pollen tube inhibition in Camellia oleifera  

作　　者：Yihong Chang Wenfang Gong Jinming Xu Han Gong Qiling Song Shixin Xiao Deyi Yuan 

机构地区：[1]Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees of the Ministry of Education and Key Laboratory of Non-Wood Forest Products of the Forestry Ministry,Central South University of Forestry and Technology,Changsha,Hunan 410004,China

出　　处：《Horticulture Research》2023年第1期260-273,共14页园艺研究（英文）

基　　金：Our work was supported by the National Key R&D Program of China(2018YFD1000603-1);the Natural Science Foundation of Hunan Province(2020JJ5968);Scientific Research Foundation for Advanced Talents of Central South University of Forestry and Technology(2018YJ002);Special Funds for the Construction of Innovative Provinces in Hunan(2021NK1007);the Key Program of Education Department of Hunan Province(grant no.20A524).

摘　　要：Camellia oil extracted from the seeds of Camellia oleifera Abel.is a popular and high-quality edible oil,but its yield is limited by seed setting,which is mainly caused by self-incompatibility(SI).One of the obvious biological features of SI plants is the inhibition of self-pollen tubes;however,the underlying mechanism of this inhibition in C.oleifera is poorly understood.In this study,we constructed a semi-in vivo pollen tube growth test(SIV-PGT)system that can screen for substances that inhibit self-pollen tubes without interference from the genetic background.Combined with multi-omics analysis,the results revealed the important role of galloylated catechins in self-pollen tube inhibition,and a possible molecular regulatory network mediated by UDP-glycosyltransferase(UGT)and serine carboxypeptidase-like(SCPL)was proposed.In summary,galloylation of catechins and high levels of galloylated catechins are specifically involved in pollen tube inhibition under self-pollination rather than cross-pollination,which provides a new understanding of SI in C.oleifera.These results will contribute to sexual reproduction research on C.oleifera and provide theoretical support for improving Camellia oil yield in production.

关 键 词：VIVO oleifera INTEGRATION 

分 类 号：S794.4[农业科学—林木遗传育种]