A splice site mutation in the FvePHP gene is associated with leaf development and f lowering time in woodland strawberry  

作　　者：Baotian Wang Weijia Li Kexin Xu Yingying Lei Di Zhao Xue Li Junxiang Zhang Zhihong Zhang 

机构地区：[1]Liaoning Key Laboratory of Strawberry Breeding and Cultivation,College of Horticulture,Shenyang Agricultural University,Shenyang,110866,China [2]Laboratory of Protected Horticulture(Shenyang Agricultural University),Ministry of Education,Shenyang,110866,China [3]Institute of Carbon Materials Science,Shanxi Datong University,Datong,037009,China [4]Analytical and Testing Center,Shenyang Agricultural University,Shenyang,110866,China

出　　处：《Horticulture Research》2023年第1期274-287,共14页园艺研究（英文）

基　　金：This work was finan-cially supported by Liao Ning Revitalization Talents Program(No.XLYC1902069).

摘　　要：Leaves and f lowers are crucial for the growth and development of higher plants.In this study we identified a mutant with narrow leaf lets and early f lowering(nlef)in an ethyl methanesulfonate-mutagenized population of woodland strawberry(Fragaria vesca)and aimed to identify the candidate gene.Genetic analysis revealed that a single recessive gene,nlef,controlled the mutant phenotype.We found that FvH4_1g25470,which encodes a putative DNA polymeraseαwith a polymerase and histidinol phosphatase domain(PHP),might be the candidate gene,using bulked segregant analysis with whole-genome sequencing,molecular markers,and cloning analyses.A splice donor site mutation(C to T)at the 5-end of the second intron led to an erroneous splice event that reduced the expression level of the full-length transcript of FvePHP in mutant plants.FvePHP was localized in the nucleus and was highly expressed in leaves.Silencing of FvePHP using the virus-induced gene silencing method resulted in partial developmental defects in strawberry leaves.Overexpression of the FvePHP gene can largely restore the mutant phenotype.The expression levels of FveSEP1,FveSEP3,FveAP1,FveFUL,and FveFT were higher in the mutants than those in‘Yellow Wonder’plants,probably contributing to the early f lowering phenotype in mutant plants.Our results indicate that mutation in FvePHP is associated with multiple developmental pathways.These results aid in understanding the role of DNA polymerase in strawberry development.

关 键 词：STRAW PHP NARROW 

分 类 号：S668.4[农业科学—果树学]