侗药黄蜡果不同部位总皂苷含量的测定  

Determination of Total Saponin Content of Different Parts of Dong Medicine Stauntonia Brachyanthera Hand.-Mazz

作　　者：舒良银 易思雷 舒琴 张小强 金苹 唐根云 Shu Liangyin;Yi Silei;Shu Qin;Zhang Xiaoqiang;Jin Ping;Tang Genyun(Hunan University of Medicine,Huaihua 418000,China;Hunan Provincial Key Laboratory of Dong Medicine,Huaihua 418000,China;Key Laboratory of Brain and Neuroendocrine Diseases of Hunan Provincial Universities,Huaihua 418000,China)

机构地区：[1]湖南医药学院,湖南怀化418000 [2]侗医药研究湖南省重点实验室,湖南怀化418000 [3]脑与神经内分泌疾病湖南省高等学校重点实验室,湖南怀化418000

出　　处：《黑龙江科学》2023年第8期96-99,共4页Heilongjiang Science

基　　金：国家自然科学基金项目(81703821);湖南省大学生创新创业训练计划项目(S202112214025);湖南医药学院组织育人项目(HYZZYR2201)。

摘　　要：为建立黄蜡果总皂苷含量的测定方法,定量评价不同部位总皂苷含量的差异,采用紫外分光光度法,测定不同部位总皂苷含量,并建立线性回归方程。结果显示,测得其叶、藤茎、根的平均总皂苷含量分别为0.701%、0.4203%、0.4937%,说明大孔树脂柱色谱能有效富集皂苷,紫外分光光度法可用于黄蜡果皂苷的定量及定性分析,特别是叶的总皂苷含量最高,这为其质量标准研究提供了科学依据。In order to establish a method for the determination of the total saponin content of the Stauntonia brachyanthera Hand.-Mazz,the study evaluates the differences in the total saponin content of different parts.The total saponin content of different parts is determined by ultraviolet spectrophotometry,and the equation of the linear regression is established.The results show that the average total saponin contents of leaves,vine stems and roots are measured as 0.701%,0.4203%and 0.4937%,respectively.Macroporous adsorptive resins can effectively enrich saponins.Ultraviolet spectrophotometry can be used for the quantitative and qualitative analysis of Stauntonia brachyanthera saponins,and the leaves have the highest total saponin content.This provides scientific basis for the study of its quality standard.

关 键 词：黄蜡果 总皂苷 含量测定 紫外分光光度法 

分 类 号：R927.2[医药卫生—药学]