水母雪莲通气组织形成的相关基因SmLSD1克隆及表达  

Cloning and Expression of Aerenchyma-related Gene SmLSD1 in Saussurea medusa Maxim

作　　者：蒋欣悦 史国民 代吴斌 李凤珍 何涛 JIANG Xinyue;SHI Guomin;DAI Wubin;LI Fengzhen;HE Tao(School of Ecol-Environmental Engineering,Qinghai University,Xining 810016,China;Key Laboratory of Landscape Plants of Qinghai Province,Xining 810016,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学生态环境工程学院,西宁810016 [2]青海省园林植物与观赏园艺重点实验室,西宁810016 [3]省部共建三江源生态与高原农牧业国家重点实验室,西宁810016

出　　处：《西北植物学报》2023年第4期550-558,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31960222)。

摘　　要：该研究以水母雪莲为实验材料,通过RT-PCR结合RACE技术克隆了通气组织形成相关基因SmLSD1(GenBank登录号为OL690334),并对该基因在不同胁迫下的表达量及编码蛋白结构进行测定分析。结果表明:(1)水母雪莲SmLSD1基因全长965 bp,包含537 bp的开放阅读框,编码178个氨基酸。(2)同源序列比对发现,水母雪莲SmLSD1蛋白与菊科植物牛蒡LSD1的氨基酸序列相似性最高,达到98.31%。(3)亚细胞定位显示SmLSD1基因主要在细胞核和细胞膜上表达;原核表达显示,SmLSD1基因编码氨基酸的分子量约为18 kD。(4)荧光定量分析显示,SmLSD1基因在根、茎、叶中均有表达,且在叶片中表达量最高;在低温、低氧及紫外胁迫下,SmLSD1基因的表达量下调。研究推测,SmLSD1基因在水母雪莲通气组织的形成以及对逆境胁迫的响应中发挥着重要作用。An aerenchyma-related gene named SmLSD1(GenBank accession number OL690334)was cloned from Saussurea medusa Maxim.with RT-PCR and RACE methods,the expression of the gene and the structure of its encoded protein under different stresses were determined and analyzed.The results showed that:(1)the length of SmLSD1 was 965 bp,contained 537 bp open reading frame and encoded 178 amino acids.(2)Homology amino acid sequence comparison showed that SmLSD1 protein had more high similarity with LSD1 in Arctium lappa(98.31%).(3)Subcellular localization assays showed that SmLSD1 protein mainly expressed in the nucleus and cell membrane;prokaryotic expression results showed that the molecular weight of the amino acid encoded by the SmLSD1 gene was about 18kD.(4)qRT-PCR showed that:SmLSD1 gene was expressed in roots,stems and leaves,and the highest expression emerged in the leaves.The expression of SmLSD1 gene was down-regulated under low temperature stress,low oxygen stress,and UV stress.It is speculated that the SmLSD1 gene played an important role in the formation of aerenchyma and in response to adverse environmental stress.

关 键 词：水母雪莲 SmLSD1基因 通气组织 表达分析 

分 类 号：Q785[生物学—分子生物学] Q786