不同来源肠炎沙门菌耐药特征及遗传多样性特点的研究  

Antibiotic resistance characteristics and genetic diversity of Salmonella Enteritidis isolated from different sources

作　　者：黄梦颖[1,2] 陈建辉 邱玉锋[1] 杨劲松 罗朝晨[1] 徐海滨[1] HUANG Meng-ying;CHEN Jian-hui;QIU Yu-feng;YANG Jin-song;LUO Chao-chen;XU Hai-bin(Fujian Center for Disease Control and Prevention,Fuzhou 350001,China;Key Laboratory of Fujian Province for Zoonotic Disease Research,Fuzhou 350001,China)

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]福建省人兽共患病研究重点实验室,福州350001

出　　处：《中国人兽共患病学报》2023年第4期318-324,332,共8页Chinese Journal of Zoonoses

基　　金：福建省卫生健康科研人才培养项目(No.2019-2-16);福建省科技创新平台建设项目(No.2019Y2001)。

摘　　要：目的分析福建地区腹泻病人和表观健康人群携带的肠炎沙门菌耐药特征及遗传多样性特点。方法收集福建地区腹泻患者和表观健康人群携带的肠炎沙门菌,采用K-B法进行药敏实验,PCR扩增毒力基因,多位点融数目亭联重复序列分析(multiple-locus variable-number tandem repeat analysis,MLVA)技术进行分子分型,并应用BioNumerics软件进行聚类分析。结果福建地区肠炎沙门菌除对CIP和NOR 100%敏感外,对其他8种药物(AMP、AMC-AC、CTX、CRO、CHL、NAL、SXT和TET)均有不同程度的耐药。腹泻患者分离株的多重耐药率(33.33%)高于表观健康人群分离株(15.22%)。10种毒力基因(sitC、hilA、sseL、sifA、mgtC、siiE、sopB、stn、spvB和pefA)均有检出,且其在腹泻患者和表观健康人群分离株中分布不同。毒力基因谱型54个(VP1~VP54),其中VP3只存在于表观健康人群分离株,且为其优势谱型,占比为13.04%;VP4和VP5只存在于腹泻患者分离株;VP4和VP3、VP1均为腹泻患者分离株中的优势谱型,占比均为11.90%。所有分离株共分为40个MLVA型(MT0001~MT0040)、2个主要基因簇(clusterⅠ和clusterⅡ),其中clusterⅡ包含的菌株均来自表观健康人群;clusterⅠ包含的菌株来自及腹泻患者和表观健康人群。结论福建地区肠炎沙门菌呈现遗传多态性,腹泻患者分离株的耐药性和致病力均高于表观健康人群分离株。We aimed to determine the antibiotic resistance characteristics and genetic diversity of Salmonella Enteritidis isolated from patients with diarrhea and healthy people in Fujian.We collected Salmonella Enteritidis from patients with diarrhea and healthy people in Fujian Province.The K-B method was used to test antimicrobial susceptibility,and PCR was used to amplify virulence genes.Multiple-locus variable-number tandem repeat analysis(MLVA)was used for molecular typing,and BioNumerics was used for cluster analysis.In addition to showing 100%sensitivity to CIP and NOR,Salmonella Enteritidis in Fujian showed different degrees of resistance to other drugs,including AMP,AMC-AC,CTX,CRO,CHL,NAL,SXT and TET.The multiple drug resistance rate of isolates from patients with diarrhea(33.33%)was higher than that from healthy people(15.22%).Ten selected virulence genes were detected,and their distribution differed between patients with diarrhea and healthy people.A total of 54 virulence gene profiles(VPs)were identified.VP3 existed in only healthy human isolates,and was the dominant virulence gene profile in isolates from healthy peo ple,accounting for 13.04%.VP4 and VP5 was observed only in patients with diarrhea,and VP4,VP3 and VP1 were the dominant virulence gene profile of patients with diarrhea,accounting for 11.90%.MLVA indicated 40 patterns in two main clusters,of which the strains in clusterⅡwere all from healthy people,and the strains in clusterⅠwere from patients with diarrhea and healthy people.Salmonella Enteritidis in Fujian showed genetic polymorphism.Isolates from patients with diarrhea showed higher drug resistance and virulence than those from healthy people.

关 键 词：肠炎沙门菌 药敏试验 毒力基因 MLVA 腹泻患者 表观健康人群 

分 类 号：R378.2[医药卫生—病原生物学]