3D细胞培养在细胞生理学研究中的应用进展  

Advances in the application of three-Dimensional cell culture in cell physiology

作　　者：王二朴 陈梦梦 张炳强 邹伟 WANG Erpu;CHEN Mengmeng;ZHANG Bingqiang;ZOU Wei(Re-store Life Institute,Qingdao Re-store Biotechnology Co.,Ltd,Qingdao 266000,China;College of Life Science,Liaoning Normal University,Dalian 116029,China)

机构地区：[1]青岛瑞思德生物科技有限公司瑞思德研究院,山东青岛266000 [2]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《实验室科学》2023年第2期1-5,共5页Laboratory Science

基　　金：青岛市科技局“百城百园”行动科技成果转化项目(2021年立项)。

摘　　要：3D细胞培养技术是生命科学中发展最快的实验技术之一。随着细胞成像和数据分析的发展以及新的细胞支架的使用,3D模式逐渐成为主流的细胞培养方式。这种培养已被证明更接近体内细胞的原始状态,能为很多研究提供有价值的细胞材料。通过介绍现阶段3D细胞培养方法、优势、现有的支架材料以及在模拟生理学相关进程中的应用,为未来的细胞生理学研究提供参考。Three-dimensional cell culture technique is one of the fastest growing experimental approaches in life sciences.Augmented with advancements in cell imaging and analytical systems,as well as the applications of new scaffolds,cells have been increasingly grown as three-dimensional models.Such cultures have proven to be closer to in vivo natural systems,thus proving to be useful material for many applications.Through the introduction of the three-dimensional way of culturing cells,their advantages,the scaffolds currently available,the applications of such cultures in modeling physiologically relevant processes,providing reference for future cell physiology research.

关 键 词：3D细胞培养 细胞支架 细胞生理学 

分 类 号：Q331[生物学—遗传学]