Optical and electrochemical dual detection ofβ-lactoglobulin based on the methylene blue@copper-based metal-organic framework  

作　　者：Yuwei Wang Jingyi Hong Xinlong Wang Liying Zhu Ling Jiang 

机构地区：[1]College of Food Science and Light Industry,State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing Tech University,Nanjing 211816,China [2]School of Chemistry and Molecular Engineering,Nanjing Tech University,Nanjing 211816,China [3]State Key Laboratory of Materials-Oriented Chemical Engineering,Nanjing Tech University,Nanjing 211816,China

出　　处：《Food Materials Research》2022年第1期131-138,共8页食材研究（英文）

基　　金：supported by the National Key R&D Program of China (2017YFC1600404);the National Natural Science Foundation of China (31922070,U2106228);the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTC2205).

摘　　要：β-lactoglobulin is an effective indicator of allergic protein detection.Herein,we produced a copper-based metal-organic framework coated with methylene blue,to realize the optical and electrochemical dual detection ofβ-lactoglobulin.Methylene blue was successfully encapsulated inside the copper-based metal-organic framework and released after addition ofβ-lactoglobulin.As the concentration ofβ-lactoglobulin increased,the intensity of the ultraviolet absorption band and the response current increased with the increasing concentration of methylene blue released from the copper-based metal-organic framework.The optical detection range is from 0.10 mg/mL to 10 mg/mL,and the detection limit is 0.10 mg/mL.The electrochemical detection range is from 1.0×10^(-7) mg/mL to 8.0×10^(-7) mg/mL,the detection limit is 2.0×10^(-8) mg/mL.The dual detection strategy,with no interfere with each other,played a synergetic role in the quick qualitative and precise quantitative analyses ofβ-lactoglobulin in a wide range of applications.

关 键 词：COPPER METHYLENE ELECTROCHEMICAL 

分 类 号：O62[理学—有机化学]